gmuC

168

glucomannan-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIC of the [category|SW.1.2.2|PTS]

locus: BSU_05830

Molecular weight: 48.27 kDa

pI: 9.8

Protein length: 442 aa

Gene length: 1329 bp

function: glucomannan uptake and phosphorylation

product: glucomannan-specific lichenan-specific [category|SW.1.2.2|PTS], EIIC component

essential: no

synonyms: gmuC, ydhO

List of homologs in different organisms, belongs to COG1455 (Galperin et al., 2021)

Gene

Coordinates: 627,284  628,612

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The protein

Protein family

• [category|SW.1.2.2|PTS] permease, lactose family [Pubmed|10627040]

[wiki|Domains]

• [wiki|PTS EIIC domain] type-3 (aa 5-411) (according to UniProt)

Structure

• [PDB|3QNQ] (EIIC component of diacetylchitobiose specific PTS from ''Bacillus cereus''; 41% identity, 80% similarity) [Pubmed|21471968]
• [AF|O05507]

Paralogous protein(s)

• [protein|83A7C160B0301A2B51B65478D99ACB753D67AF74|licC], [protein|0650C9EAB395EB6ABDADBE72F0DA9F8E5E606B0E|ywbA]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

additional information

• [protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• MGNA-C190 (ydhO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2188 NBRP B. subtilis, Japan]
• BKE05830 ([gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAACACCCTATCCACCCCGA,  downstream forward: _UP4_ATGTGACATAAGGGGAGAGA
• BKK05830 ([gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAACACCCTATCCACCCCGA,  downstream forward: _UP4_ATGTGACATAAGGGGAGAGA

References

• 18177310,10627040,20817675,21471968