Gmk

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  • Description: guanylate kinase (GMP:dATP, dGMP:ATP)

Gene name gmk
Synonyms yloD
Essential yes PubMed
Product guanylate kinase (GMP:dATP, dGMP:ATP)
Function GTP biosynthesis
Gene expression levels in SubtiExpress: gmk
Metabolic function and regulation of this protein in SubtiPathways:
gmk
MW, pI 23 kDa, 4.616
Gene length, protein length 612 bp, 204 aa
Immediate neighbours ylzA, yloH
Sequences Protein DNA DNA_with_flanks
Genetic context
Gmk context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Gmk expression.png



















Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU15680

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + GMP = ADP + GDP (according to Swiss-Prot)
  • Protein family: guanylate kinase-like domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-134 and/or Arg-149PubMed
  • Cofactor(s):
  • Effectors of protein activity:
    • inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´PubMed

Database entries

  • Structure: 1S4Q (from Mycobacterium tuberculosis h37rv, 39% identity, 60% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 254 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1208 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References