GlyS

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  • Description: glycyl-tRNA synthetase (beta subunit)

Gene name glyS
Synonyms yqfK
Essential yes PubMed
Product glycyl-tRNA synthetase (beta subunit)
Function translation
Gene expression levels in SubtiExpress: glyS
Interactions involving this protein in SubtInteract: GlyS
Metabolic function and regulation of this protein in SubtiPathways:
glyS
MW, pI 76 kDa, 4.973
Gene length, protein length 2037 bp, 679 aa
Immediate neighbours ccpN, glyQ
Sequences Protein DNA DNA_with_flanks
Genetic context
GlyS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlyS expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU25260

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly) (according to Swiss-Prot)
  • Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1J5W (from Thermotoga maritima, 55% identity, 72% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 693 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2795 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1055 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 922 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 982 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Audrey R Nelson, Tina M Henkin, Paul F Agris
tRNA regulation of gene expression: interactions of an mRNA 5'-UTR with a regulatory tRNA.
RNA: 2006, 12(7);1254-61
[PubMed:16741230] [WorldCat.org] [DOI] (P p)

Mary R Yousef, Frank J Grundy, Tina M Henkin
Structural transitions induced by the interaction between tRNA(Gly) and the Bacillus subtilis glyQS T box leader RNA.
J Mol Biol: 2005, 349(2);273-87
[PubMed:15890195] [WorldCat.org] [DOI] (P p)

Frank J Grundy, Mary R Yousef, Tina M Henkin
Monitoring uncharged tRNA during transcription of the Bacillus subtilis glyQS gene.
J Mol Biol: 2005, 346(1);73-81
[PubMed:15663928] [WorldCat.org] [DOI] (P p)

Frank J Grundy, Tina M Henkin
Kinetic analysis of tRNA-directed transcription antitermination of the Bacillus subtilis glyQS gene in vitro.
J Bacteriol: 2004, 186(16);5392-9
[PubMed:15292140] [WorldCat.org] [DOI] (P p)