malP

168

maltose-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIICB of the [category|SW.1.2.2|PTS]

locus: BSU_08200

Molecular weight: 57.89 kDa

pI: 8.87

Protein length: 527 aa

Gene length: 1584 bp

function: maltose uptake and phosphorylation

product: maltose-specific [category|SW.1.2.2|PTS], EIICB

essential: no

ec: 2.7.1.69

synonyms: malP, glvC

List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)

Gene

Coordinates: 892,215 → 893,798

Phenotypes of a mutant

• no growth on maltose as single carbon source [Pubmed|10627040]

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The protein

Catalyzed reaction/ biological activity

• D-maltose + N^π-phospho-L-histidyl-[protein] --> α-maltose 6'-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• [category|SW.1.2.2|PTS] permease, glucose family [Pubmed|10627040]

[wiki|Domains]

• [wiki|PTS EIIB domain] type-1 (aa 449–527) (according to UniProt)
• [wiki|PTS EIIC domain] type-1 (aa 1-418) (according to UniProt)

Structure

• [PDB|6BVG] (EIIC domain from B. cereus, corresponds to aa 6 - 419, 27% identity) [pubmed|29784777]
• [AF|P54715]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-C291 (glvC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2289 NBRP B. subtilis, Japan]
• GP110 (spc), available in [wiki|Stülke] lab
• BKE08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC,  downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC
• BKK08200 (Δ[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTGCATCATACCAGAACCCC,  downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC

References

• 16707683,10627040,11489864,30038046,29784777