Difference between revisions of "GltC"

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(Biological materials)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' GP344 (erm), GP738 (spc)
+
* '''Mutant:''' GP344 (erm), GP738 (spc)(available in Stülke lab)
  
* '''Expression vector:''' pGP903 (N-terminal His-tag)
+
* '''Expression vector:''' pGP903 (N-terminal His-tag) (available in Stülke lab)
 
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 17:42, 12 December 2008

  • Synonyms:
  • Description: Transcriptional activator of the gltAB operon. Activates expression of the operon in the absence of arginine.


The gene

Basic information

  • Coordinates:
  • Gene length:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry:[2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activation of the gltAB operon
  • Protein family: LysR-type transcription regulator
  • Paralogous protein(s): none, but there are 19 members of the LysR family in B. subtilis

Extended information on the protein

  • Kinetic information:
  • Domains: DNA-binding helix-turn-helix motif: AA 18 ... 37
  • Modification:
  • Effectors of protein activity: 2-oxoglutarate
  • Interactions:
    • GltC-RocG, This interaction takes place in the presence of glutamate. It prevents the transcription activation of the gltAB operon. Note that RocG expression is strongly regulated. <ref>Commichau et al. (2007)</ref>
  • Localization:

Database entries

  • Structure:

Additional information

Expression and regulation

  • Operon: gltC
  • Sigma factor: SigA
  • Regulation: autoregulation by GltC
  • Regulatory mechanism: autorepression
  • Additional information:

Biological materials

  • Mutant: GP344 (erm), GP738 (spc)(available in Stülke lab)
  • Expression vector: pGP903 (N-terminal His-tag) (available in Stülke lab)
  • lacZ fusion:
  • GFP fusion:
  • Antibody: available in Stülke lab

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA [3]

Jörg Stülke, University of Göttingen, Germany [4]

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. [5]