GlsA

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  • Description: glutaminase, low affinity for glutamine

Gene name glsA
Synonyms ybgJ
Essential no
Product glutaminase
Function glutamine degradation
Gene expression levels in SubtiExpress: glsA
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 36 kDa, 5.315
Gene length, protein length 981 bp, 327 aa
Immediate neighbours glnT, glnK
Sequences Protein DNA Advanced_DNA
Genetic context
YbgJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlsA expression.png




























Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

GlnL regulon

The gene

Basic information

  • Locus tag: BSU02430

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: l-glutamine + H2O = L-glutamate + NH3 (according to Swiss-Prot)
  • Protein family: glutaminase family (according to Swiss-Prot)
  • Paralogous protein(s): YlaM

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of glutamine (GlnL) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Greg Brown, Alex Singer, Michael Proudfoot, Tatiana Skarina, Youngchang Kim, Changsoo Chang, Irina Dementieva, Ekaterina Kuznetsova, Claudio F Gonzalez, Andrzej Joachimiak, Alexei Savchenko, Alexander F Yakunin
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis.
Biochemistry: 2008, 47(21);5724-35
[PubMed:18459799] [WorldCat.org] [DOI] (I p)

Takenori Satomura, Daisuke Shimura, Kei Asai, Yoshito Sadaie, Kazutake Hirooka, Yasutaro Fujita
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
J Bacteriol: 2005, 187(14);4813-21
[PubMed:15995196] [WorldCat.org] [DOI] (P p)