Difference between revisions of "GlsA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || glutamine degradation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || glutamine degradation
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.315   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.315   

Revision as of 10:34, 17 June 2009

  • Description: glutaminase, low affinity for glutamine

Gene name glsA
Synonyms ybgJ
Essential no
Product glutaminase
Function glutamine degradation
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 36 kDa, 5.315
Gene length, protein length 981 bp, 327 aa
Immediate neighbours glnT, glnK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbgJ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU02430

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: l-glutamine + H2O = L-glutamate + NH3 (according to Swiss-Prot)
  • Protein family: glutaminase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Takenori Satomura, Daisuke Shimura, Kei Asai, Yoshito Sadaie, Kazutake Hirooka, Yasutaro Fujita
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
J Bacteriol: 2005, 187(14);4813-21
[PubMed:15995196] [WorldCat.org] [DOI] (P p)