glsA

168

glutaminase, low affinity for glutamine

locus: BSU_02430

Molecular weight: 36.03 kDa

pI: 5.32

Protein length: 327 aa

Gene length: 984 bp

function: glutamine degradation

product: glutaminase

essential: no

ec: 3.5.1.2

synonyms: glsA, ybgJ

List of homologs in different organisms, belongs to COG2066 (Galperin et al., 2021)

Gene

Coordinates: 264,191  265,174

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The protein

Catalyzed reaction/ biological activity

• H₂O + L-glutamine --> L-glutamate + NH₄⁺ (according to UniProt)

Protein family

• glutaminase family (with [protein|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM], according to UniProt)

Structure

• [PDB|3BRM] [PDB|1MKI][Pubmed|18459799]
• [AF|O31465]

Paralogous protein(s)

• [protein|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]

Biological materials

Mutant

• GP4253 (Δ[gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]::''cat''), available in [wiki|Jörg Stülke]'s lab
• MGNA-B943 (ybgJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1942 NBRP B. subtilis, Japan]
• BKE02430 ([gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGAATTCCTCCTCACTG,  downstream forward: _UP4_TAAACATGAAAAAAGAGGTG
• BKK02430 ([gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGAATTCCTCCTCACTG,  downstream forward: _UP4_TAAACATGAAAAAAGAGGTG

References

• 15995196,18459799