Difference between revisions of "GlpT"

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(Expression and regulation)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' QB5437, available in the [[Stülke]] lab
+
* '''Mutant:''' QB5437 (ermC), available in the [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 17:26, 2 September 2009

  • Description: glycerol-3-phosphate permease

Gene name glpT
Synonyms ybeE
Essential no
Product glycerol-3-phosphate permease
Function glycerol-3-phosphate uptake
MW, pI 49 kDa, 9.366
Gene length, protein length 1332 bp, 444 aa
Immediate neighbours glpQ, ybeF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GlpT context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU02140

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: SLC37A family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (6.2-fold) (CcpA) PubMed
    • induced by glycerol (GlpP) PubMed
    • induced by phosphate limitation
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: QB5437 (ermC), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

R P Nilsson, L Beijer, B Rutberg
The glpT and glpQ genes of the glycerol regulon in Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 4);723-30
[PubMed:8012593] [WorldCat.org] [DOI] (P p)