GlpD

• Description: glycerol-3-phosphate dehydrogenase (menaquinone 7)
  
  

Gene name	glpD
Synonyms
Essential	no
Product	glycerol-3-phosphate dehydrogenase (menaquinone 7)
Function	glycerol utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	62 kDa, 8.002
Gene length, protein length	1665 bp, 555 aa
Immediate neighbours	glpK, pgcA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU09300

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol (according to Swiss-Prot)

• Protein family: FAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): FAD

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3DA1 (from Bacillus halodurans, 57% identity, 72% similarity)

• UniProt: P18158

• KEGG entry: [3]

• E.C. number: 1.1.5.3

Expression and regulation

• Operon: glpD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA)
  • induced by glycerol (GlpP) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • GlpP: transcriptional antitermination at a RNA switch PubMed

• Additional information:

Biological materials

• Mutant: QB5347 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

E Glatz, A Farewell, B Rutberg
The Bacillus subtilis glpD leader and antiterminator protein GlpP provide a target for glucose repression in Escherichia coli.
FEMS Microbiol Lett: 1998, 162(1);93-6
[PubMed:9595668] [WorldCat.org] [DOI] (P p)

Elisabeth Glatz, Martin Persson, Blanka Rutberg
Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA.
Microbiology (Reading): 1998, 144 ( Pt 2);449-456
[PubMed:9493382] [WorldCat.org] [DOI] (P p)

E Glatz, R P Nilsson, L Rutberg, B Rutberg
A dual role for the Bacillus subtilis glpD leader and the GlpP protein in the regulated expression of glpD: antitermination and control of mRNA stability.
Mol Microbiol: 1996, 19(2);319-28
[PubMed:8825777] [WorldCat.org] [DOI] (P p)

C Holmberg, L Rutberg
An inverted repeat preceding the Bacillus subtilis glpD gene is a conditional terminator of transcription.
Mol Microbiol: 1992, 6(20);2931-8
[PubMed:1479885] [WorldCat.org] [DOI] (P p)