glmM
168
phosphoglucosamine mutase, required for cell wall synthesis, inhibits [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] activity
locus
BSU_01770
Molecular weight
48.27 kDa
pI
4.68
function
cell wall synthesis, control of c-di-AMP synthesis
product
phosphoglucosamine mutase, required for cell wall synthesis
essential
yes
ec
5.4.2.10
synonyms
glmM, ybbT
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1109 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
198,497 → 199,843
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
D-glucosamine 6-phosphate --> α-D-glucosamine 1-phosphate (according to UniProt)
inhibition of [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] activity ([pubmed|34678313]) under conditions of osmotic stress (demonstrated in L. monocytogenes ) [pubmed|32250026]
Protein family
phosphohexose mutase family (with [protein|70412A747D1ED3E885126945DF02503667C13E5B|pgcA], [pubmed|15238632])
Structure
[PDB|7OML]
[PDB|7OLH], [PDB|7OJS] (complex of the [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] [wiki|DAC domain] and [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM])
[PDB|3PDK] (from ''B. anthracis'') [Pubmed|21685296]
[AF|O34824]
Modification
phosphorylation on Ser-100 [Pubmed|17218307,17726680,20097688], this is an autophosphorylation [Pubmed|20097688]
Biological materials
Expression vectors
pGP400: (expression in ''B. subtilis'' in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pGP1401: (expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP1402: (cloning vector for glmM (GlmM S100A) in ''E. coli'', in [wiki|pBlueskript KS]), available in [wiki|Jörg Stülke]'s lab
pGP1403: (expression of GlmM (S100A) in ''B. subtilis'' in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pGP1405: (expression, purification of GlmM (S100A) in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1380 ''lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC'' (based on [wiki|pGP1460] and [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
GP1340 (cat) based on [wiki|pAC6], available in [wiki|Jörg Stülke]'s lab [pubmed|23192352]
Antibody
available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1382 ''glmM-gfp ermC'' (based on [wiki|pGP1080]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-04-24 08:49:18
Author of last update: Jstuelk