glmM

168

phosphoglucosamine mutase, required for cell wall synthesis, inhibits [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] activity

locus: BSU_01770

Molecular weight: 48.27 kDa

pI: 4.68

Protein length: 448 aa

Gene length: 1347 bp

function: cell wall synthesis, control of c-di-AMP synthesis

product: phosphoglucosamine mutase, required for cell wall synthesis

essential: yes

ec: 5.4.2.10

synonyms: glmM, ybbT

List of homologs in different organisms, belongs to COG1109 (Galperin et al., 2021)

Gene

Coordinates: 198,497 → 199,843

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• D-glucosamine 6-phosphate --> α-D-glucosamine 1-phosphate (according to UniProt)
• inhibition of [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] activity ([pubmed|34678313]) under conditions of osmotic stress (demonstrated in L. monocytogenes ) [pubmed|32250026]

Protein family

• phosphohexose mutase family (with [protein|70412A747D1ED3E885126945DF02503667C13E5B|pgcA], [pubmed|15238632])

Structure

• [PDB|7OML]
• [PDB|7OLH], [PDB|7OJS] (complex of the [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] [wiki|DAC domain] and [protein|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM])
• [PDB|3PDK] (from ''B. anthracis'') [Pubmed|21685296]
• [AF|O34824]

Modification

• phosphorylation on Ser-100 [Pubmed|17218307,17726680,20097688], this is an autophosphorylation  [Pubmed|20097688]

Biological materials

Expression vectors

• pGP400: (expression in ''B. subtilis'' in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
• pGP1401: (expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
• pGP1402: (cloning vector for glmM (GlmM S100A) in ''E. coli'', in [wiki|pBlueskript KS]), available in [wiki|Jörg Stülke]'s lab
• pGP1403: (expression of GlmM (S100A) in ''B. subtilis'' in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
• pGP1405: (expression, purification of GlmM (S100A) in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

FLAG-tag construct

• GP1380 ''lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC'' (based on [wiki|pGP1460] and [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab

lacZ fusion

• GP1340 (cat) based on [wiki|pAC6], available in [wiki|Jörg Stülke]'s lab [pubmed|23192352]

Antibody

• available in [wiki|Jörg Stülke]'s lab

GFP fusion

• GP1382 ''glmM-gfp ermC'' (based on [wiki|pGP1080]), available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 28683921,18266853,37954098

Original Publications

• 32250026,17726680,17218307,8550580,10231382,10671448,20097688,20525796,21685296,23192352,22211522,26240071,26585449,15238632,30668586,31589605,34678313