glgA

168

glycogen synthase (ADPGlc)

locus: BSU_30950

Molecular weight: 55.69 kDa

pI: 5.16

Protein length: 484 aa

Gene length: 1455 bp

function: glycogen biosynthesis

product: glycogen synthase (ADPGlc)

essential: no

ec: 2.4.1.21

synonyms: glgA

List of homologs in different organisms, belongs to COG0297 (Galperin et al., 2021)

Gene

Coordinates: 3,166,118 → 3,167,572

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The protein

Catalyzed reaction/ biological activity

• [(1→4)-α-D-glucosyl]_(n) + ADP-α-D-glucose --> [(1→4)-α-D-glucosyl]_(n+1) + ADP + H⁺ (according to UniProt)

Protein family

• glycosyltransferase 1 family (single member, according to UniProt)

Structure

• [PDB|2QZS] (from E. coli, 37% identity) [pubmed|19244233]
• [AF|P39125]

Biological materials

Mutant

• BKE30950 (Δ[gene|EBEA85AD543B79C321CB5F28D1B7E1248640DC1D|glgA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30950 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCTGACACAGCAAATAATA,  downstream forward: _UP4_GAGCAGGTGACAAGGAGTGG
• BKK30950 (Δ[gene|EBEA85AD543B79C321CB5F28D1B7E1248640DC1D|glgA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30950 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCTGACACAGCAAATAATA,  downstream forward: _UP4_GAGCAGGTGACAAGGAGTGG

References

• 8145641,19244233