Difference between revisions of "GlcT"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' available in [[Stülke]] lab:
+
* '''Mutant:''' available in [[Jörg Stülke]]'s lab:
 
** GP109 (in frame deletion)
 
** GP109 (in frame deletion)
** GP778 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette)
+
** GP777 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette), {{PubMed|22722928}}
** GP926 (substitution of ''[[glcT]]'' and ''[[ptsG]]'' by a tet cassette)
+
** GP926 (substitution of ''[[glcT]]'' and ''[[ptsG]]'' by a tet cassette), {{PubMed|22722928}}
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  
** pGP124 (full length, in [[pWH844]]), available in [[Stülke]] lab
+
** pGP124 (full length, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
** pGP114 (amino acids 1-60, RNA-binding domain, in [[pWH844]]), available in [[Stülke]] lab
+
** pGP114 (amino acids 1-60, RNA-binding domain, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
** pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [[pWH844]]), available in [[Stülke]] lab
+
** pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
** pGP164 (both PRDs, in [[pWH844]]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [[pWH844]]), available in [[Stülke]] lab
+
** pGP164 (both PRDs, in [[pWH844]]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [[pWH844]]), available in [[Jörg Stülke]]'s lab
** pGP424 (PRDI, in [[pWH844]]), available in [[Stülke]] lab
+
** pGP424 (PRDI, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
** pGP425 (PRDII, in [[pWH844]]), available in [[Stülke]] lab
+
** pGP425 (PRDII, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
** pGP442 (PRDI, in [[pGP570]], with thrombin cleavage site), available in [[Stülke]] lab
+
** pGP442 (PRDI, in [[pGP570]], with thrombin cleavage site), available in [[Jörg Stülke]]'s lab
** pGP443 (PRDII, in [[pGP570]],  with thrombin cleavage site), available in [[Stülke]] lab  
+
** pGP443 (PRDII, in [[pGP570]],  with thrombin cleavage site), available in [[Jörg Stülke]]'s lab  
** pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [[pGP574]]), available in [[Stülke]] lab
+
** pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [[pGP574]]), available in [[Jörg Stülke]]'s lab
 
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  
* '''GFP fusion:''' GP1224 (spc, based on [[pGP1870]]), available in the [[Stülke]] lab
+
* '''GFP fusion:''' GP1224 (spc, based on [[pGP1870]]), available in [[Jörg Stülke]]'s lab
  
* '''YFP fusion:''' GP1228 (spc, based on [[pGP1871]]), available in the [[Stülke]] lab
+
* '''YFP fusion:''' GP1228 (spc, based on [[pGP1871]]), available in [[Jörg Stülke]]'s lab
  
* '''FLAG-tag construct:''' GP1220 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
+
* '''FLAG-tag construct:''' GP1220 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 08:28, 3 September 2013

Gene name glcT
Synonyms ykwA
Essential no
Product transcriptional antiterminator of the ptsG-ptsH-ptsI operon
Function control of glucose uptake
Gene expression levels in SubtiExpress: glcT
Interactions involving this protein in SubtInteract: GlcT
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 33,0 kDa, 7.01
Gene length, protein length 855 bp, 285 amino acids
Immediate neighbours ykvZ, ptsG
Sequences Protein DNA DNA_with_flanks
Genetic context
GlcT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlcT expression.png
















Categories containing this gene/protein

carbon core metabolism, transcription factors and their control, RNA binding regulators, phosphoproteins

This gene is a member of the following regulons

The GlcT regulon: ptsG-ptsH-ptsI

The gene

Basic information

  • Locus tag: BSU13880

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

Extended information on the protein

  • Kinetic information:
  • Domains:
    • RNA-binding domain (N-terminal, constitutive antiterminator)
    • 2x PTS regulation domains (PRDs) (C-terminal, neg. regulated by PtsG)
  • Modification: phosphorylation (His104)
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • lacZ fusion:
  • Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

Reviews

Fabian M Commichau, Jörg Stülke
Trigger enzymes: bifunctional proteins active in metabolism and in controlling gene expression.
Mol Microbiol: 2008, 67(4);692-702
[PubMed:18086213] [WorldCat.org] [DOI] (P p)

J Stülke, M Arnaud, G Rapoport, I Martin-Verstraete
PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria.
Mol Microbiol: 1998, 28(5);865-74
[PubMed:9663674] [WorldCat.org] [DOI] (P p)

Original publications

Sebastian Himmel, Christian Grosse, Sebastian Wolff, Claudia Schwiegk, Stefan Becker
Structure of the RBD-PRDI fragment of the antiterminator protein GlcT.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2012, 68(Pt 7);751-6
[PubMed:22750856] [WorldCat.org] [DOI] (I p)

Sebastian Himmel, Christopher P Zschiedrich, Stefan Becker, He-Hsuan Hsiao, Sebastian Wolff, Christine Diethmaier, Henning Urlaub, Donghan Lee, Christian Griesinger, Jörg Stülke
Determinants of interaction specificity of the Bacillus subtilis GlcT antitermination protein: functionality and phosphorylation specificity depend on the arrangement of the regulatory domains.
J Biol Chem: 2012, 287(33);27731-42
[PubMed:22722928] [WorldCat.org] [DOI] (I p)

Sebastian Himmel, Sebastian Wolff, Stefan Becker, Donghan Lee, Christian Griesinger
Detection and identification of protein-phosphorylation sites in histidines through HNP correlation patterns.
Angew Chem Int Ed Engl: 2010, 49(47);8971-4
[PubMed:20939030] [WorldCat.org] [DOI] (I p)

Dayté D Rodríguez, Christian Grosse, Sebastian Himmel, César González, Iñaki M de Ilarduya, Stefan Becker, George M Sheldrick, Isabel Usón
Crystallographic ab initio protein structure solution below atomic resolution.
Nat Methods: 2009, 6(9);651-3
[PubMed:19684596] [WorldCat.org] [DOI] (I p)

Oliver Schilling, Christina Herzberg, Tina Hertrich, Hanna Vörsmann, Dirk Jessen, Sebastian Hübner, Fritz Titgemeyer, Jörg Stülke
Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples.
Nucleic Acids Res: 2006, 34(21);6102-15
[PubMed:17074746] [WorldCat.org] [DOI] (I p)

Oliver Schilling, Ines Langbein, Michael Müller, Matthias H Schmalisch, Jörg Stülke
A protein-dependent riboswitch controlling ptsGHI operon expression in Bacillus subtilis: RNA structure rather than sequence provides interaction specificity.
Nucleic Acids Res: 2004, 32(9);2853-64
[PubMed:15155854] [WorldCat.org] [DOI] (I e)

Matthias H Schmalisch, Steffi Bachem, Jörg Stülke
Control of the Bacillus subtilis antiterminator protein GlcT by phosphorylation. Elucidation of the phosphorylation chain leading to inactivation of GlcT.
J Biol Chem: 2003, 278(51);51108-15
[PubMed:14527945] [WorldCat.org] [DOI] (P p)

David B Greenberg, Jorg Stülke, Milton H Saier
Domain analysis of transcriptional regulators bearing PTS regulatory domains.
Res Microbiol: 2002, 153(8);519-26
[PubMed:12437213] [WorldCat.org] [DOI] (P p)

I Langbein, S Bachem, J Stülke
Specific interaction of the RNA-binding domain of the bacillus subtilis transcriptional antiterminator GlcT with its RNA target, RAT.
J Mol Biol: 1999, 293(4);795-805
[PubMed:10543968] [WorldCat.org] [DOI] (P p)

S Bachem, J Stülke
Regulation of the Bacillus subtilis GlcT antiterminator protein by components of the phosphotransferase system.
J Bacteriol: 1998, 180(20);5319-26
[PubMed:9765562] [WorldCat.org] [DOI] (P p)

J Stülke, I Martin-Verstraete, M Zagorec, M Rose, A Klier, G Rapoport
Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT.
Mol Microbiol: 1997, 25(1);65-78
[PubMed:11902727] [WorldCat.org] [DOI] (P p)