Difference between revisions of "GlcT"

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(Biological materials)
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* '''Mutant:''' GP109 (in frame deletion), available in [[Stülke]] lab
 
* '''Mutant:''' GP109 (in frame deletion), available in [[Stülke]] lab
  
* '''Expression vector:''' pGP124 (full length, in [[pWH844]]), pGP114 (amino acids 1-60, RNA-binding domain, in [[pWH844]]), pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [[pWH844]]), pGP164 (both PRDs, in [[pWH844]]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [[pWH844]]), pGP424 (PRDI, in [[pWH844]]), pGP425 (PRDII, in [[pWH844]]), pGP442 (PRDI, in [[pGP570]], with thrombin cleavage site), pGP443 (PRDII, in [[pGP570]],  with thrombin cleavage site), pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [[pGP574]]), all available in [[Stülke]] lab
+
* '''Expression vector:'''  
 +
** pGP124 (full length, in [[pWH844]]), available in [[Stülke]] lab
 +
** pGP114 (amino acids 1-60, RNA-binding domain, in [[pWH844]]), available in [[Stülke]] lab
 +
** pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [[pWH844]]), available in [[Stülke]] lab
 +
** pGP164 (both PRDs, in [[pWH844]]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [[pWH844]]), available in [[Stülke]] lab
 +
** pGP424 (PRDI, in [[pWH844]]), available in [[Stülke]] lab
 +
** pGP425 (PRDII, in [[pWH844]]), available in [[Stülke]] lab
 +
** pGP442 (PRDI, in [[pGP570]], with thrombin cleavage site), available in [[Stülke]] lab
 +
** pGP443 (PRDII, in [[pGP570]],  with thrombin cleavage site), available in [[Stülke]] lab
 +
** pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [[pGP574]]), available in [[Stülke]] lab
 
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 15:43, 2 September 2009

  • Description: Transcriptional antiterminator, controls expression of the ptsGHI operon

Gene name glcT
Synonyms ykwA
Essential no
Product transcriptional antiterminator of the ptsGHI operon
Function transcriptional antiterminator
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 33,0 kDa, 7.01
Gene length, protein length 855 bp, 285 amino acids
Immediate neighbours ykvZ, ptsG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GlcT context.gif
This image was kindly provided by SubtiList








The gene

Basic information

  • Locus tag: BSU13880

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcriptional antiterminator, RNA-binding protein, binds the ptsG RAT sequence
  • Protein family: antiterminator of the BglG/ SacY family

Extended information on the protein

  • Kinetic information:
  • Domains:
    • RNA-binding domain (N-terminal, constitutive antiterminator)
    • 2x PTS regulation domains (PRDs) (C-terminal, neg. regulated by PtsG)
  • Modification: phosphorylation (His104)
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP109 (in frame deletion), available in Stülke lab
  • Expression vector:
    • pGP124 (full length, in pWH844), available in Stülke lab
    • pGP114 (amino acids 1-60, RNA-binding domain, in pWH844), available in Stülke lab
    • pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in pWH844), available in Stülke lab
    • pGP164 (both PRDs, in pWH844), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in pWH844), available in Stülke lab
    • pGP424 (PRDI, in pWH844), available in Stülke lab
    • pGP425 (PRDII, in pWH844), available in Stülke lab
    • pGP442 (PRDI, in pGP570, with thrombin cleavage site), available in Stülke lab
    • pGP443 (PRDII, in pGP570, with thrombin cleavage site), available in Stülke lab
    • pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in pGP574), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References