Difference between revisions of "GlcK"
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Revision as of 11:18, 16 April 2012
- Description: glucose kinase (D-glucose:ATP)
| Gene name | glcK |
| Synonyms | yqgR |
| Essential | no |
| Product | glucose kinase (D-glucose:ATP) |
| Function | phosphorylation of the free glucose moiety resulting from cleavage of di-and oligosaccharides |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism | |
| MW, pI | 33 kDa, 5.988 |
| Gene length, protein length | 963 bp, 321 aa |
| Immediate neighbours | yqgS, yqgQ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
utilization of specific carbon sources, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU24850
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + D-glucose = ADP + D-glucose 6-phosphate (according to Swiss-Prot)
- Protein family: ROK (nagC/xylR) family (according to Swiss-Prot)
- Paralogous protein(s): XylR
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure: 2QM1 (from Enterococcus faecalis, 38% identity, 52% similarity)
- UniProt: P54495
- KEGG entry: [2]
- E.C. number: 2.7.1.2
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP2 (spc), available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Stefan Schönert, Sabine Seitz, Holger Krafft, Eva-Anne Feuerbaum, Iris Andernach, Gabriele Witz, Michael K Dahl
Maltose and maltodextrin utilization by Bacillus subtilis.
J Bacteriol: 2006, 188(11);3911-22
[PubMed:16707683]
[WorldCat.org]
[DOI]
(P p)
Lili R Mesak, Felix M Mesak, Michael K Dahl
Expression of a novel gene, gluP, is essential for normal Bacillus subtilis cell division and contributes to glucose export.
BMC Microbiol: 2004, 4;13
[PubMed:15050034]
[WorldCat.org]
[DOI]
(I e)
Lili R Mesak, Felix M Mesak, Michael K Dahl
Bacillus subtilis GlcK activity requires cysteines within a motif that discriminates microbial glucokinases into two lineages.
BMC Microbiol: 2004, 4;6
[PubMed:15018644]
[WorldCat.org]
[DOI]
(I e)
P Skarlatos, M K Dahl
The glucose kinase of Bacillus subtilis.
J Bacteriol: 1998, 180(12);3222-6
[PubMed:9620975]
[WorldCat.org]
[DOI]
(P p)
F Titgemeyer, J Reizer, A Reizer, M H Saier
Evolutionary relationships between sugar kinases and transcriptional repressors in bacteria.
Microbiology (Reading): 1994, 140 ( Pt 9);2349-54
[PubMed:7952186]
[WorldCat.org]
[DOI]
(P p)
