gerKA

168

nutrient receptor

locus: BSU_03700

Molecular weight: 60.22 kDa

pI: 5.21

Protein length: 544 aa

Gene length: 1635 bp

function: germination response to the combination of glucose, fructose, aspartate, and KCl

product: part of the putative nutrient-gated [protein|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[protein|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]-[protein|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC] ion channel

essential: no

synonyms: gerKA

List of homologs in different organisms, belongs to COG5901 (Galperin et al., 2021)

Gene

Coordinates: 420,110 → 421,744

Advertisement

The protein

Catalyzed reaction/ biological activity

• the [protein|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]-[protein|D1AFCB1BE693AB6B8461B7764B6641E6EB1404FA|gerKB]-[protein|D5D4E1E182F972BEF6BB432A7FF25EF45F1F0D95|gerKC] ion channel likely releases ions from the spore core in response to asparagine, glucose, fructose and K⁺ to trigger [category|SW.4.2.4|Germination], this requires the gerB locus [pubmed|37104613]

Protein family

• [wiki|GerABKA family] (according to UniProt)

[wiki|Domains]

• contains multiple membrane-spanning domains [Pubmed|23335419]

Structure

• [PDB|6O59] (from B. megaterium, corresponds to aa 40 ... 299, 53.6% identity) [pubmed|31113879]
• [AF|P49939]

Paralogous protein(s)

• [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA], [protein|AE28299B0F274B189DD90554A5A4F7B9A9479B60|yndD], [protein|3368743E6E03792DB83A38A19989123304DF7560|gerBA], [protein|7CE0CAF27B1C0F507E783B8B80622270B00634FD|yfkQ]

[wiki|Localization]

• outer surface of the inner spore membrane [Pubmed|23335419,21696470]

Additional information

• 700 molecules are present per spore [Pubmed|23749970]

Biological materials

Mutant

• BKE03700 (Δ[gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACACAAATACCTTTCCT,  downstream forward: _UP4_AAAGATTTGGAAGAAGGGGA
• BKK03700 (Δ[gene|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03700 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACACAAATACCTTTCCT,  downstream forward: _UP4_AAAGATTTGGAAGAAGGGGA

References

• 36815589,16707705,16352818,21696470,23335419,23396907,22343299,16740944,23749970,24752279,26731423,31113879,37104613