GerD

• Description: required for clustering of germinant receptors in the spore inner membrane
  
  

Gene name	gerD
Synonyms
Essential	no
Product	lipoprotein
Function	germination
Gene expression levels in SubtiExpress: gerD
Interactions involving this protein in SubtInteract: GerD
MW, pI	20 kDa, 4.862
Gene length, protein length	555 bp, 185 aa
Immediate neighbours	salA, kbaA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigF regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU01550

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: required for clustering of germinant receptors in the spore inner membrane PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GerD is part of the germinosome in the spore inner membrane PubMed
  • GerD-Lgt to fix GerD to diacylglycerol in the membrane PubMed

• Localization:
  • outer surface of the inner spore membrane PubMed
  • inner spore membrane, spore coat, cortex, germ cell wall, some GerD is also present in the soluble fraction PubMed

Database entries

• Structure:

• UniProt: P16450

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gerD PubMed

• Expression browser: gerD PubMed

• Sigma factor: SigF PubMed, SigG PubMed

• Regulation:
  • expression level depends on sporulation conditions (medim composition, temperature) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A677 ( gerD::erm), PubMed, available at BGSC
  • 1G17 ( gerD::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Anne Moir lab

Labs working on this gene/protein

Anne Moir, University of Sheffield, UK

References

Additional references: PubMed

George Korza, Peter Setlow
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.
J Bacteriol: 2013, 195(7);1484-91
[PubMed:23335419] [WorldCat.org] [DOI] (I p)

Kerry-Ann V Stewart, Xuan Yi, Sonali Ghosh, Peter Setlow
Germination protein levels and rates of germination of spores of Bacillus subtilis with overexpressed or deleted genes encoding germination proteins.
J Bacteriol: 2012, 194(12);3156-64
[PubMed:22493018] [WorldCat.org] [DOI] (I p)

Arturo Ramirez-Peralta, Pengfei Zhang, Yong-Qing Li, Peter Setlow
Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores.
Appl Environ Microbiol: 2012, 78(8);2689-97
[PubMed:22327596] [WorldCat.org] [DOI] (I p)

Xuan Yi, Jintao Liu, James R Faeder, Peter Setlow
Synergism between different germinant receptors in the germination of Bacillus subtilis spores.
J Bacteriol: 2011, 193(18);4664-71
[PubMed:21725007] [WorldCat.org] [DOI] (I p)

Keren K Griffiths, Jingqiao Zhang, Ann E Cowan, Ji Yu, Peter Setlow
Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.
Mol Microbiol: 2011, 81(4);1061-77
[PubMed:21696470] [WorldCat.org] [DOI] (I p)

Wiyada Mongkolthanaruk, Carl Robinson, Anne Moir
Localization of the GerD spore germination protein in the Bacillus subtilis spore.
Microbiology (Reading): 2009, 155(Pt 4);1146-1151
[PubMed:19332816] [WorldCat.org] [DOI] (P p)

Patricia L Pelczar, Peter Setlow
Localization of the germination protein GerD to the inner membrane in Bacillus subtilis spores.
J Bacteriol: 2008, 190(16);5635-41
[PubMed:18556788] [WorldCat.org] [DOI] (I p)

Patricia L Pelczar, Takao Igarashi, Barbara Setlow, Peter Setlow
Role of GerD in germination of Bacillus subtilis spores.
J Bacteriol: 2007, 189(3);1090-8
[PubMed:17122337] [WorldCat.org] [DOI] (P p)

E H Kemp, R L Sammons, A Moir, D Sun, P Setlow
Analysis of transcriptional control of the gerD spore germination gene of Bacillus subtilis 168.
J Bacteriol: 1991, 173(15);4646-52
[PubMed:1906867] [WorldCat.org] [DOI] (P p)

J R Yon, R L Sammons, D A Smith
Cloning and sequencing of the gerD gene of Bacillus subtilis.
J Gen Microbiol: 1989, 135(12);3431-45
[PubMed:2517635] [WorldCat.org] [DOI] (P p)