GcvT

From SubtiWiki
Revision as of 11:42, 8 April 2014 by 134.76.70.252 (talk)
Jump to: navigation, search
  • Description: aminomethyltransferase (glycine cleavage system protein T)

Gene name gcvT
Synonyms yqhI
Essential no
Product aminomethyltransferase
(glycine cleavage system protein T)
Function glycine utilization
Gene expression levels in SubtiExpress: gcvT
Metabolic function and regulation of this protein in SubtiPathways:
GcvT
MW, pI 39 kDa, 5.734
Gene length, protein length 1086 bp, 362 aa
Immediate neighbours gcvPA, yqhH
Sequences Protein DNA DNA_with_flanks
Genetic context
GcvT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GcvT expression.png















Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

Gly-box

The gene

Basic information

  • Locus tag: BSU24570

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: [Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3 (according to Swiss-Prot)
  • Protein family: gcvT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
    • tetrahydrofolate
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP431 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kokoro Hamachi, Hikari Hayashi, Miyuki Shimamura, Yuiha Yamaji, Ai Kaneko, Aruma Fujisawa, Takuya Umehara, Koji Tamura
Glycols modulate terminator stem stability and ligand-dependency of a glycine riboswitch.
Biosystems: 2013, 113(2);59-65
[PubMed:23721735] [WorldCat.org] [DOI] (I p)

Nathan J Baird, Adrian R Ferré-D'Amaré
Modulation of quaternary structure and enhancement of ligand binding by the K-turn of tandem glycine riboswitches.
RNA: 2013, 19(2);167-76
[PubMed:23249744] [WorldCat.org] [DOI] (I p)

Maumita Mandal, Mark Lee, Jeffrey E Barrick, Zasha Weinberg, Gail Mitchell Emilsson, Walter L Ruzzo, Ronald R Breaker
A glycine-dependent riboswitch that uses cooperative binding to control gene expression.
Science: 2004, 306(5694);275-9
[PubMed:15472076] [WorldCat.org] [DOI] (I p)

Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624] [WorldCat.org] [DOI] (P p)