Difference between revisions of "GcvT"

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(References)
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|style="background:#ABCDEF;" align="center"|'''Function''' || glycine utilization  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || glycine utilization  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU24570 gcvT]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24570 gcvT]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 5.734   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 5.734   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gcvPA]]'', ''[[yqhH]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gcvPA]]'', ''[[yqhH]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14388&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24570 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24570 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24570 Advanced_DNA]
 
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Revision as of 13:16, 13 May 2013

  • Description: aminomethyltransferase (glycine cleavage system protein T)

Gene name gcvT
Synonyms yqhI
Essential no
Product aminomethyltransferase
(glycine cleavage system protein T)
Function glycine utilization
Gene expression levels in SubtiExpress: gcvT
MW, pI 39 kDa, 5.734
Gene length, protein length 1086 bp, 362 aa
Immediate neighbours gcvPA, yqhH
Sequences Protein DNA Advanced_DNA
Genetic context
GcvT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GcvT expression.png



























Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

Gly-box

The gene

Basic information

  • Locus tag: BSU24570

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: [Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3 (according to Swiss-Prot)
  • Protein family: gcvT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP431 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Nathan J Baird, Adrian R Ferré-D'Amaré
Modulation of quaternary structure and enhancement of ligand binding by the K-turn of tandem glycine riboswitches.
RNA: 2013, 19(2);167-76
[PubMed:23249744] [WorldCat.org] [DOI] (I p)

Maumita Mandal, Mark Lee, Jeffrey E Barrick, Zasha Weinberg, Gail Mitchell Emilsson, Walter L Ruzzo, Ronald R Breaker
A glycine-dependent riboswitch that uses cooperative binding to control gene expression.
Science: 2004, 306(5694);275-9
[PubMed:15472076] [WorldCat.org] [DOI] (I p)

Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624] [WorldCat.org] [DOI] (P p)