gcaD

168

bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

locus: BSU_00500

Molecular weight: 49.29 kDa

pI: 5.65

Protein length: 456 aa

Gene length: 1371 bp

function: cell wall metabolism

product: bifunctional N-acetylglucosamine-1-phosphate

essential: yes

ec: 2.7.7.23

synonyms: gcaD, tms, tms26, glmU

List of homologs in different organisms, belongs to COG1207 (Galperin et al., 2021)

Gene

Coordinates: 56,352  57,722

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + α-D-glucosamine 1-phosphate --> CoA + H⁺ + N-acetyl-α-D-glucosamine 1-phosphate (according to UniProt)
• H⁺ + N-acetyl-α-D-glucosamine 1-phosphate + UTP --> diphosphate + UDP-N-acetyl-α-D-glucosamine (according to UniProt)

Protein family

• N-terminal domain: N-acetylglucosamine-1-phosphate uridyltransferase family (single member, according to UniProt)
• C-terminal domain: [wiki|Transferase hexapeptide repeat family] (according to UniProt)

Structure

• [PDB|1HM9] (from ''Streptococcus pneumoniae'', 49% identity) [Pubmed|11118459]
• [AF|P14192]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Expression vectors

• pGP2596: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab

References

• 1328164,8522540,11118459,21303765