GbsB

• Description: choline dehydrogenase (FAD-dependent), glycine betaine synthesis
  
  

Gene name	gbsB
Synonyms
Essential	no
Product	choline dehydrogenase (FAD-dependent)
Function	osmoprotection
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	43 kDa, 4.912
Gene length, protein length	1209 bp, 403 aa
Immediate neighbours	yuaD, gbsA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

miscellaneous metabolic pathways, coping with hyper-osmotic stress

The gene

Basic information

• Locus tag: BSU31050

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: An alcohol + NAD⁺ = an aldehyde or ketone + NADH (according to Swiss-Prot)

• Protein family: iron-containing alcohol dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P71017

• KEGG entry: [2]

• E.C. number: 1.1.1.1

Expression and regulation

• Operon: gbsA-gbsB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by choline (YuaC) PubMed

• Regulatory mechanism:
  • YuaC: transcription repression evidence

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

J Boch, B Kempf, R Schmid, E Bremer
Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes.
J Bacteriol: 1996, 178(17);5121-9
[PubMed:8752328] [WorldCat.org] [DOI] (P p)