gbsB

168

choline dehydrogenase (FAD-dependent), glycine betaine synthesis

locus: BSU_31050

Molecular weight: 43.39 kDa

pI: 4.91

Protein length: 403 aa

Gene length: 1209 bp

function: osmoprotection

product: choline dehydrogenase (FAD-dependent)

essential: no

ec: 1.1.1.1

synonyms: gbsB

List of homologs in different organisms, belongs to COG1454 (Galperin et al., 2021)

Gene

Coordinates: 3,183,867 → 3,185,075

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The protein

Catalyzed reaction/ biological activity

• choline + NAD⁺ --> betaine aldehyde + H⁺ + NADH (according to UniProt)

Protein family

• iron-containing alcohol dehydrogenase family (with [protein|1E951C2B640966643D5F081159522CD1C51942C8|yugJ] and [protein|D81610C7CA2A92FCB1195591ACB613E7C1288F6B|yugK], according to UniProt)

[wiki|Cofactors]

• FAD

Structure

• [PDB|3BFJ] (from Klebsiella pneumoniae, 39% identity) [pubmed|19011020]
• [AF|P71017]

Biological materials

Mutant

• 1A1056 ( ''gbsB''::''kan''), [Pubmed|21296969], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1056&Search=1A1056 BGSC]
• BKE31050 (Δ[gene|B90D4E798145F2AF3421DDC5AB6CD578E2E17900|gbsB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAATGTCATCATCAATCTCC,  downstream forward: _UP4_TAATCAAAATCCCGCTCCTT
• BKK31050 (Δ[gene|B90D4E798145F2AF3421DDC5AB6CD578E2E17900|gbsB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAATGTCATCATCAATCTCC,  downstream forward: _UP4_TAATCAAAATCCCGCTCCTT

labs

• [wiki|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

• 8752328,21296969,22408163,29159878,19011020