GbsA

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  • Description: glycine betaine-aldehyde dehydrogenase, glycine betaine synthesis

Gene name gbsA
Synonyms
Essential no
Product glycine betaine-aldehyde dehydrogenase
Function osmoprotection
Gene expression levels in SubtiExpress: gbsA
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 53 kDa, 4.713
Gene length, protein length 1470 bp, 490 aa
Immediate neighbours gbsB, gbsR
Sequences Protein DNA DNA_with_flanks
Genetic context
GbsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GbsA expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, coping with hyper-osmotic stress

This gene is a member of the following regulons

GbsR regulon

The gene

Basic information

  • Locus tag: BSU31060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Betaine aldehyde + NAD+ + H2O = betaine + NADH (according to Swiss-Prot)
  • Protein family: aldehyde dehydrogenase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 31% identity, 67% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Gabriele Nau-Wagner, Daniela Opper, Anne Rolbetzki, Jens Boch, Bettina Kempf, Tamara Hoffmann, Erhard Bremer
Genetic control of osmoadaptive glycine betaine synthesis in Bacillus subtilis through the choline-sensing and glycine betaine-responsive GbsR repressor.
J Bacteriol: 2012, 194(10);2703-14
[PubMed:22408163] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

J Boch, G Nau-Wagner, S Kneip, E Bremer
Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine.
Arch Microbiol: 1997, 168(4);282-9
[PubMed:9297465] [WorldCat.org] [DOI] (P p)

J Boch, B Kempf, R Schmid, E Bremer
Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes.
J Bacteriol: 1996, 178(17);5121-9
[PubMed:8752328] [WorldCat.org] [DOI] (P p)