Difference between revisions of "GbsA"

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(Basic information/ Evolution)
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1470 bp, 490 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1470 bp, 490 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gbsB]]'', ''[[yuaC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gbsB]]'', ''[[gbsR]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15084&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15084&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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* '''Regulation:'''  
 
* '''Regulation:'''  
** induced by choline ([[YuaC]]) {{PubMed|8752328}}
+
** induced by choline ([[GbsR]]) {{PubMed|22408163,8752328}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[YuaC]]: transcription repression [http://archiv.ub.uni-marburg.de/diss/z2009/0477/pdf/ddo.pdf evidence]
+
** [[GbsR]]: transcription repression {{PubMed|22408163}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
 
+
'''Additional publications:''' {{PubMed|22408163}}
 
<pubmed>9297465,8752328, 21296969 </pubmed>
 
<pubmed>9297465,8752328, 21296969 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:29, 15 March 2012

  • Description: glycine betaine-aldehyde dehydrogenase, glycine betaine synthesis

Gene name gbsA
Synonyms
Essential no
Product glycine betaine-aldehyde dehydrogenase
Function osmoprotection
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 53 kDa, 4.713
Gene length, protein length 1470 bp, 490 aa
Immediate neighbours gbsB, gbsR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GbsA context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

miscellaneous metabolic pathways, coping with hyper-osmotic stress

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU31060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Betaine aldehyde + NAD+ + H2O = betaine + NADH (according to Swiss-Prot)
  • Protein family: aldehyde dehydrogenase family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 31% identity, 67% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

Your additional remarks

References

Additional publications: PubMed

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

J Boch, G Nau-Wagner, S Kneip, E Bremer
Glycine betaine aldehyde dehydrogenase from Bacillus subtilis: characterization of an enzyme required for the synthesis of the osmoprotectant glycine betaine.
Arch Microbiol: 1997, 168(4);282-9
[PubMed:9297465] [WorldCat.org] [DOI] (P p)

J Boch, B Kempf, R Schmid, E Bremer
Synthesis of the osmoprotectant glycine betaine in Bacillus subtilis: characterization of the gbsAB genes.
J Bacteriol: 1996, 178(17);5121-9
[PubMed:8752328] [WorldCat.org] [DOI] (P p)