Difference between revisions of "GapB"

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=Biological materials =
 
=Biological materials =
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* '''Mutant:'''
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* '''Expression vector:'''
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* '''lacZ fusion:'''
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* '''GFP fusion:'''
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* '''Antibody:'''
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 12:48, 14 January 2009

  • Description: glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent, gluconeogenic enzyme

Gene name gapB
Synonyms ppc
Essential no
Product glyceraldehyde-3-phosphate dehydrogenase2
Function anabolic enzyme in gluconeogenesis
MW, pI 37,3 kDa, 6.47
Gene length, protein length 1020 bp, 340 amino acids
Immediate neighbours ytcD, speD
Gene sequence (+200bp) Protein sequence
Genetic context
GapB context.gif



The gene

Basic information

  • Coordinates: 2966075 - 2967094

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
  • Protein family: glyceraldehyde-3-phosphate dehydrogenase family
  • Paralogous protein(s): GapA

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Nucleotid binding Domain (12-13)
    • 2x Glyceraldehyde 3-phosphate binding Domain (151-153) & (210-211)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm

Database entries

  • Structure:
  • Swiss prot entry: [3]
  • KEGG entry: [4]
  • E.C. number: [5]

Additional information

Expression and regulation

  • Operon:
  • Regulation: repressed (70-times) by Glc, repressor CcpN PubMed
  • Regulatory mechanism: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References

  1. Meile JC, Wu LJ, Ehrlich SD (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6(7): 2135-46. PubMed
  2. Servant et al. (2005) CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes. Mol. Microbiol. 55: 1435-1451. PubMed
  3. Tännler et al. (2008) CcpN controls central carbon fluxes in Bacillus subtilis. J. Bacteriol. 190: 6178-6187. PubMed
  4. Thomaides, H. B., Davison, E. J., Burston, L., Johnson, H., Brown, D. R., Hunt, A. C., Errington, J., and Czaplewski, L. (2007) Essential bacterial functions encoded by gene pairs. J Bacteriol 189, 591-602. PubMed