Difference between revisions of "GapA"

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(Expression and regulation)
(Expression and regulation)
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The primary mRNAs of the operon are highly unstable. The primary mRNA is subject to processing at the very end of the [[''cggR'']] open reading frame. This results in stable mature ''[[gapA]]'' and ''[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' mRNAs.  
 
The primary mRNAs of the operon are highly unstable. The primary mRNA is subject to processing at the very end of the [[''cggR'']] open reading frame. This results in stable mature ''[[gapA]]'' and ''[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' mRNAs.  
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GapA is one of the most abundant proteins in the cell. In the presence of glucose, there are about 25,000 GapA molecules per cell.
  
 
* '''Sigma factor:''' SigA  
 
* '''Sigma factor:''' SigA  

Revision as of 17:14, 12 December 2008

  • Synonyms:
  • Description: glyceraldehyde-3-phosphate dehydrogenase, (NADH-dependent), glycolytic enzyme

The gene

Basic information

  • Coordinates:
  • Gene length:

Phenotypes of a mutant

essential

Database entries

  • DBTBS entry: [1]
  • SubtiList entry:[2]

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: glyceraldehyde-3-phosphate dehydrogenase, (NADH-dependent)
  • Protein family:
  • Paralogous protein(s): GapB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: Phosphorylation (STY)
  • Effectors of protein activity:
  • Interactions:
    • GapA-PtsH: HPr(Ser-46-P) binds GapA resulting in a slight inhibition of enzymatic activity.
    • GapA-Crh: Crh(Ser-46-P) binds GapA resulting in a slight inhibition of enzymatic activity.
  • Localization: membrane and cytoplasm

Database entries

  • Structure:
    • 3CMC (from Geobacillus stearothermophilus)
    • 1NQO (from Geobacillus stearothermophilus, mutant with cys 149 replaced by ser, complex with NAD+ und D-Glyceraldehyde-3-Phosphate)

Expression and regulation

The primary mRNAs of the operon are highly unstable. The primary mRNA is subject to processing at the very end of the ''cggR'' open reading frame. This results in stable mature gapA and gapA-pgk-tpiA-pgm-eno mRNAs.

GapA is one of the most abundant proteins in the cell. In the presence of glucose, there are about 25,000 GapA molecules per cell.

  • Sigma factor: SigA
  • Regulation: CggR represses the operon in the absence of glycolytic sugars
  • Regulatory mechanism: repression

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:


Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. [3]