Difference between revisions of "GapA"

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(Database entries)
(Database entries)
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** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NQO 1NQO] (from ''Geobacillus stearothermophilus'', mutant with cys 149 replaced by ser, complex with NAD+ und D-Glyceraldehyde-3-Phosphate)
 
** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NQO 1NQO] (from ''Geobacillus stearothermophilus'', mutant with cys 149 replaced by ser, complex with NAD+ und D-Glyceraldehyde-3-Phosphate)
  
* '''Swiss prot entry:'''
+
* '''Swiss prot entry:''' [http://www.expasy.ch/cgi-bin/sprot-search-ac?P09124 P09124]
  
 
* '''KEGG entry:'''
 
* '''KEGG entry:'''

Revision as of 17:01, 12 December 2008

  • Synonyms:
  • Description: glyceraldehyde-3-phosphate dehydrogenase, (NADH-dependent), glycolytic enzyme

The gene

Basic information

  • Coordinates:
  • Gene length:

Phenotypes of a mutant

essential

Database entries

  • DBTBS entry: [1]
  • SubtiList entry:[2]

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: glyceraldehyde-3-phosphate dehydrogenase, (NADH-dependent)
  • Protein family:
  • Paralogous protein(s): GapB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: Phosphorylation (STY)
  • Effectors of protein activity:
  • Interactions:
    • GapA-PtsH: HPr(Ser-46-P) binds GapA resulting in a slight inhibition of enzymatic activity.
    • GapA-Crh: Crh(Ser-46-P) binds GapA resulting in a slight inhibition of enzymatic activity.
  • Localization: membrane and cytoplasm

Database entries

  • Structure:
    • 3CMC (from Geobacillus stearothermophilus)
    • 1NQO (from Geobacillus stearothermophilus, mutant with cys 149 replaced by ser, complex with NAD+ und D-Glyceraldehyde-3-Phosphate)
  • KEGG entry:

Expression and regulation

  • Regulation: CggR represses the operon in the absence of glycolytic sugars
  • Regulatory mechanism: repression

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:


Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. [3]