Difference between revisions of "GanA"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
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* '''Mutant:'''GP1181 (aphA3) available in the [[Stülke]] lab
 +
 
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 16:13, 7 February 2011

  • Description: beta-galactosidase

Gene name lacA
Synonyms yvfN
Essential no
Product beta-galactosidase
Function galactan utilization
MW, pI 79 kDa, 5.704
Gene length, protein length 2061 bp, 687 aa
Immediate neighbours yvfO, yvfM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LacA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

  • Locus tag: BSU34130

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 42 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:GP1181 (aphA3) available in the Stülke lab


  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Stephanie Shipkowski, Jean E Brenchley
Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases.
Appl Environ Microbiol: 2006, 72(12);7730-8
[PubMed:17056685] [WorldCat.org] [DOI] (P p)

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)

J Errington, C H Vogt
Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator.
J Bacteriol: 1990, 172(1);488-90
[PubMed:2104611] [WorldCat.org] [DOI] (P p)