GamP

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Gene name gamP
Synonyms ybfS, yzfA
Essential no
Product glucosamine-specific phosphotransferase system, EIICBA
Function glucosamine uptake and phosphorylation
Gene expression levels in SubtiExpress: gamP
Interactions involving this protein in SubtInteract: GamP
Metabolic function and regulation of this protein in SubtiPathways:
gamP
MW, pI 67 kDa, 5.472
Gene length, protein length 1893 bp, 631 aa
Immediate neighbours gltP, gamA
Gene sequence (+200bp) Protein sequence
Genetic context
GamP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GamP expression.png















Categories containing this gene/protein

cell wall degradation/ turnover, phosphotransferase systems, utilization of specific carbon sources, membrane proteins, phosphoproteins

This gene is a member of the following regulons

GamR regulon

The gene

Basic information

  • Locus tag: BSU02350

Phenotypes of a mutant

delayed growth with glucosamine as the single carbon source PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
  • Protein family: PTS permease, glucose permease (Glc) family PubMed
  • Paralogous protein(s): NagP

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: QB6098 (aphA3), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Isabelle Gaugué, Jacques Oberto, Jacqueline Plumbridge
Regulation of amino sugar utilization in Bacillus subtilis by the GntR family regulators, NagR and GamR.
Mol Microbiol: 2014, 92(1);100-15
[PubMed:24673833] [WorldCat.org] [DOI] (I p)

Isabelle Gaugué, Jacques Oberto, Harald Putzer, Jacqueline Plumbridge
The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine.
PLoS One: 2013, 8(5);e63025
[PubMed:23667565] [WorldCat.org] [DOI] (I e)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)