GamP

• Description: glucosamine-specific phosphotransferase system, EIICBA of the PTS
  
  

Gene name	gamP
Synonyms	ybfS, yzfA
Essential	no
Product	glucosamine-specific phosphotransferase system, EIICBA
Function	glucosamine uptake and phosphorylation
Gene expression levels in SubtiExpress: gamP
Interactions involving this protein in SubtInteract: GamP
Metabolic function and regulation of this protein in SubtiPathways: gamP
MW, pI	67 kDa, 5.472
Gene length, protein length	1893 bp, 631 aa
Immediate neighbours	gltP, gamA
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, phosphotransferase systems, utilization of specific carbon sources, membrane proteins, phosphoproteins

This gene is a member of the following regulons

GamR regulon

The gene

Basic information

• Locus tag: BSU02350

Phenotypes of a mutant

delayed growth with glucosamine as the single carbon source PubMed

Database entries

• BsubCyc: BSU02350

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

• Protein family: PTS permease, glucose permease (Glc) family PubMed

• Paralogous protein(s): NagP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions: GamP-HPr

• Localization: membrane PubMed

Database entries

• BsubCyc: BSU02350

• Structure:

• UniProt: P39816

• KEGG entry: [2]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: gamA-gamP PubMed

• Expression browser: gamP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by glucosamine (GamR) PubMed

• Regulatory mechanism:
  • GamR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: QB6098 (aphA3), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: