gamA

168

glucosamine-6-phosphate deaminase

locus: BSU_02360

Molecular weight: 27.14 kDa

pI: 5.79

Protein length: 249 aa

Gene length: 750 bp

function: glucosamine utilization

product: glucosamine-6-phosphate deaminase

essential: no

ec: null

synonyms: gamA, ybfT

List of homologs in different organisms, belongs to COG0363 (Galperin et al., 2021)

Gene

Coordinates: 256,823  257,572

Phenotypes of a mutant

• no growth on glucosamine [Pubmed|23667565]

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The protein

Catalyzed reaction/ biological activity

• α-D-glucosamine 6-phosphate + H₂O --> β-D-fructose 6-phosphate + NH₄⁺ (according to UniProt)

Protein family

• glucosamine/galactosamine-6-phosphate isomerase family (with [protein|0C8D7EAC2656E989E45B5B7E42FAA6D258956B56|nagB], according to UniProt)

Structure

• [PDB|2BKX] (the structure of [protein|0C8D7EAC2656E989E45B5B7E42FAA6D258956B56|nagB], 50% identity) [Pubmed|15755726]
• [AF|O31458]

Kinetic information

• K(M): 3.0 mM [Pubmed|14343123]

Paralogous protein(s)

• [protein|0C8D7EAC2656E989E45B5B7E42FAA6D258956B56|nagB]

Biological materials

Mutant

• MGNA-B938 (ybfT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1937 NBRP B. subtilis, Japan]
• BKE02360 ([gene|8BBC9AFF4CD56A3A66E270A2BBA193D095EE01D1|gamA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGACACCCCCTCAAAG,  downstream forward: _UP4_TAACACAATAAAAGGAGACT
• BKK02360 ([gene|8BBC9AFF4CD56A3A66E270A2BBA193D095EE01D1|gamA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGACACCCCCTCAAAG,  downstream forward: _UP4_TAACACAATAAAAGGAGACT

References

• 10627040,14343123,23667565,23876412,24673833,15755726