gamA

gamA
168

glucosamine-6-phosphate deaminase

locus
BSU_02360
Molecular weight
27.14 kDa
pI
5.79
Protein length
Gene length
function
glucosamine utilization
product
glucosamine-6-phosphate deaminase
essential
no
ec
null
synonyms
gamA, ybfT

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0363 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
256,823  257,572
Phenotypes of a mutant
no growth on glucosamine [Pubmed|23667565]
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The protein
Catalyzed reaction/ biological activity
α-D-glucosamine 6-phosphate + H2O --> β-D-fructose 6-phosphate + NH4+ (according to UniProt)
Protein family
glucosamine/galactosamine-6-phosphate isomerase family (with [protein|0C8D7EAC2656E989E45B5B7E42FAA6D258956B56|nagB], according to UniProt)
Structure
[PDB|2BKX] (the structure of [protein|0C8D7EAC2656E989E45B5B7E42FAA6D258956B56|nagB], 50% identity) [Pubmed|15755726]
[AF|O31458]
Kinetic information
K(M): 3.0 mM [Pubmed|14343123]
Paralogous protein(s)
[protein|0C8D7EAC2656E989E45B5B7E42FAA6D258956B56|nagB]
Expression and Regulation
Operons
genes
[gene|8BBC9AFF4CD56A3A66E270A2BBA193D095EE01D1|gamA]-[gene|4DF1740D4DFB9FD25E77C14D28AEA01707776099|gamP]
description
[Pubmed|23667565]
regulation
induced by glucosamine ([protein|search|GamR]) [Pubmed|24673833,23667565]
regulatory mechanism
[protein|FB074E8CFCA1C0198457170A7AFD85C875B34DF2|gamR]: repression, [Pubmed|24673833,23667565], in [regulon|protein:FB074E8CFCA1C0198457170A7AFD85C875B34DF2|gamR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23667565], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|8BBC9AFF4CD56A3A66E270A2BBA193D095EE01D1|gamA]→[gene|4DF1740D4DFB9FD25E77C14D28AEA01707776099|gamP]

2024-03-12 00:18:48

ghost

41

4fffcf05befb972653171bbbe342dd9b2c49f8b3

1347DE6686FBBF7ED2CBD5C47BA6DFFC67338C93

Biological materials
Mutant
MGNA-B938 (ybfT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1937 NBRP B. subtilis, Japan]
BKE02360 ([gene|8BBC9AFF4CD56A3A66E270A2BBA193D095EE01D1|gamA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGACACCCCCTCAAAG,  downstream forward: _UP4_TAACACAATAAAAGGAGACT
BKK02360 ([gene|8BBC9AFF4CD56A3A66E270A2BBA193D095EE01D1|gamA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGACACCCCCTCAAAG,  downstream forward: _UP4_TAACACAATAAAAGGAGACT
References
10627040,14343123,23667565,23876412,24673833,15755726

8BBC9AFF4CD56A3A66E270A2BBA193D095EE01D1

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Time of last update: 2024-03-28 20:00:06

Author of last update: Melvin.boenninger