Difference between revisions of "GabR"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03890&redirect=T BSU03890]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gabR.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gabR.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03890&redirect=T BSU03890]
  
 
* '''Structure:'''  
 
* '''Structure:'''  

Revision as of 12:59, 2 April 2014

Gene name gabR
Synonyms ycnF
Essential no
Product transcription regulator
Function regulation of gamma-amino butyric acid utilization
Gene expression levels in SubtiExpress: gabR
MW, pI 54 kDa, 6.709
Gene length, protein length 1437 bp, 479 aa
Immediate neighbours yczG, gabT
Sequences Protein DNA DNA_with_flanks
Genetic context
GabR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GabR expression.png















Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

GabR regulon

The GabR regulon:

The gene

Basic information

  • Locus tag: BSU03890

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • transcription activation of the gabT-gabD operon in the presence of GABA and PLP, transcription repression of gabR PubMed
  • Paralogous protein(s): none, but there are 7 members of the MocR/ GabR family in B. subtilis

Extended information on the protein

  • Kinetic information:
  • Domains:
    • N-terminal DNA-binding helix-turn-helix motif (corresponding to domains of the GntR family) PubMed
    • C-terminal domain is homologous to PLP-binding large domain of aminotransferases PubMed
  • Modification:
  • Effectors of protein activity:
    • activation of gabT-gabD expression is triggered by gamma-amino butyrate and pyridoxalphosphate PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
    • GabR: negative autoregulation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Your additional remarks

References

Raji Edayathumangalam, Rui Wu, Roman Garcia, Yuguang Wang, Wei Wang, Cheryl A Kreinbring, Alicia Bach, Jingling Liao, Todd A Stone, Thomas C Terwilliger, Quyen Q Hoang, Boris R Belitsky, Gregory A Petsko, Dagmar Ringe, Dali Liu
Crystal structure of Bacillus subtilis GabR, an autorepressor and transcriptional activator of gabT.
Proc Natl Acad Sci U S A: 2013, 110(44);17820-5
[PubMed:24127574] [WorldCat.org] [DOI] (I p)

E Bramucci, T Milano, S Pascarella
Genomic distribution and heterogeneity of MocR-like transcriptional factors containing a domain belonging to the superfamily of the pyridoxal-5'-phosphate dependent enzymes of fold type I.
Biochem Biophys Res Commun: 2011, 415(1);88-93
[PubMed:22020104] [WorldCat.org] [DOI] (I p)

Boris R Belitsky
Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator.
J Mol Biol: 2004, 340(4);655-64
[PubMed:15223311] [WorldCat.org] [DOI] (P p)

Boris R Belitsky, Abraham L Sonenshein
GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis.
Mol Microbiol: 2002, 45(2);569-83
[PubMed:12123465] [WorldCat.org] [DOI] (P p)

Sébastien Rigali, Adeline Derouaux, Fabrizio Giannotta, Jean Dusart
Subdivision of the helix-turn-helix GntR family of bacterial regulators in the FadR, HutC, MocR, and YtrA subfamilies.
J Biol Chem: 2002, 277(15);12507-15
[PubMed:11756427] [WorldCat.org] [DOI] (P p)