gabD

168

succinate-semialdehyde dehydrogenase (NADP), general stress protein

locus: BSU_03910

Molecular weight: 50.10 kDa

pI: 4.76

Protein length: 462 aa

Gene length: 1389 bp

function: utilization of gamma-amino butyric acid

product: succinate-semialdehyde dehydrogenase (NADP)

essential: no

synonyms: gabD, ycnH

List of homologs in different organisms, belongs to COG1012 (Galperin et al., 2021)

Gene

Coordinates: 442,950  444,338

Phenotypes of a mutant

• growth defect in the presence of gamma-aminobutyrate  [Pubmed|24127574]

Advertisement

The protein

Catalyzed reaction/ biological activity

• H₂O + NADP⁺ + succinate semialdehyde --> 2 H⁺ + NADPH + succinate (according to UniProt)

Protein family

• [wiki|aldehyde dehydrogenase family] (according to UniProt)

Structure

• [PDB|3RHH] (from from ''Bacillus halodurans'' C-125 complexed with NADP, 36% identity, 68% similarity)
• [AF|P94428]

Paralogous protein(s)

• [protein|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT], [protein|F3341F205CB939498109D2A54DE842065C488DD5|aldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|aldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC], [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|dhaS], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA], [protein|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA], [protein|99F1FAF28FB4817D94E84BD5288FA33124558933|ywdH]

Biological materials

Mutant

• MGNA-C015 (ycnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2013 NBRP B. subtilis, Japan]
• BKE03910 ([gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGAACATTCCTTTCT,  downstream forward: _UP4_TAAAAGAATGCACGCTCCTG
• BKK03910 ([gene|A0BEB92D54799956A4ADE106A1388E5710141069|gabD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGAACATTCCTTTCT,  downstream forward: _UP4_TAAAAGAATGCACGCTCCTG

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

labs

• [wiki|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

References

• 15223311,12123465,11544224,15805528,28516784