Difference between revisions of "FsrA"

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(→‎The RNA)
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* '''Description:''' regulatory RNA<br/><br/>
+
* '''Description:''' fructokinase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''fsrA''
+
|''fruC''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''mrgC''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydjE ''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || sRNA FsrA
+
|style="background:#ABCDEF;" align="center"| '''Product''' || fructokinase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || inhibition of ''[[sdhC]]'' translation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of sucrose and glucitol
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length''' || aaa bp
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 4.623 
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuI]]'', ''[[ykuJ]]''
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 960 bp, 320 aa
 
|-
 
|-
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gutP]]'', ''[[pspA]]''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:FsrA_context.jpg]]
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12436&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:ydjE_context.gif]]
 +
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
 
|}
 
|}
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=== Basic information ===
 
=== Basic information ===
  
−
* '''Coordinates (CDS):'''
+
* '''Locus tag:'''
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
−
* '''SubtiList entry:''' no entry
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12796]
  
−
* '''Structure:'''
+
=== Additional information===
 +
 
 +
 
 +
=The protein=
 +
 
 +
=== Basic information/ Evolution ===
  
−
=== Additional information===
+
* '''Catalyzed reaction/ biological activity:''' Fru ----> Fru-6-P
  
−
* '''Swiss prot entry:'''
+
* '''Protein family:''' carbohydrate kinase pfkB family (according to Swiss-Prot)
  
−
* '''KEGG entry:''' no entry
+
* '''Paralogous protein(s):'''
−
 
 
−
* '''E.C. number:'''
 
  
−
=== Additional information===
+
=== Extended information on the protein ===
  
−
=The RNA=
+
* '''Kinetic information:'''
  
−
=== Basic information/ Evolution ===
+
* '''Domains:'''
  
−
* '''Catalyzed reaction/ biological activity:''' hybridizes to ''[[sdhC]], [[citB]], [[lutB]], and [[leuC]]-[[leuD]]'' mRNAs and inhibits their translation. [http://www.ncbi.nlm.nih.gov/sites/entrez/18697947 PubMed]
+
* '''Modification:'''
  
−
=== Extended information on the RNA===
+
* '''Cofactor(s):'''
  
−
* '''Kinetic information'''
+
* '''Effectors of protein activity:'''
  
−
* '''Interactions:''' Binds ''[[sdhC]], [[citB]], [[lutB]], and [[leuC]]-[[leuD]]'' mRNAs [http://www.ncbi.nlm.nih.gov/sites/entrez/18697947 PubMed]
+
* '''Interactions:'''
  
−
* '''Localization'''
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''Structure''':  
+
* '''Structure:'''
 +
 
 +
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34768 O34768]
 +
 
 +
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU06170]
 +
 
 +
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
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* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
−
* '''Regulation:''' induced by iron starvation ([[Fur]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18697947 PubMed]
+
* '''Regulation:''' constitutively expressed
  
−
* '''Regulatory mechanism:''' [[Fur]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/18697947 PubMed]
+
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
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* '''Expression vector:'''
 
* '''Expression vector:'''
−
+
       
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  
−
=Labs working on this gene/RNA=
+
* '''GFP fusion:'''
 +
 
 +
* '''two-hybrid system:'''
 +
 
 +
* '''Antibody:'''
  
−
[[John Helmann]], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
+
=Labs working on this gene/protein=
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
−
# Gaballa A, Antelmann H, Aguilar C, Khakh SK, Song KB, Smaldone GT, Helmann JD (2008) The ''Bacillus subtilis'' iron-sparing response is mediated by a Fur-regulated small RNA and three small, basic proteins. PNAS 105: 11927-11932. [http://www.ncbi.nlm.nih.gov/sites/entrez/18697947 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 14:42, 2 June 2009

  • Description: fructokinase

Gene name fruC
Synonyms ydjE
Essential no
Product fructokinase
Function utilization of sucrose and glucitol
MW, pI 34 kDa, 4.623
Gene length, protein length 960 bp, 320 aa
Immediate neighbours gutP, pspA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YdjE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Fru ----> Fru-6-P
  • Protein family: carbohydrate kinase pfkB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: constitutively expressed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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