frlB

168

fructoselysine-6-P-glycosidase

locus: BSU_32610

Molecular weight: 36.72 kDa

pI: 5.44

Protein length: 328 aa

Gene length: 987 bp

function: metabolism of aminoacylated fructose

product: fructoselysine-6-P-glycosidase

essential: no

synonyms: frlB, yurP

List of homologs in different organisms, belongs to COG2222 (Galperin et al., 2021)

Gene

Coordinates: 3,351,110 3,352,096

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The protein

[wiki|Domains]

• 2 [wiki|SIS domain]s (aa 15-153, aa 181-311) (according to UniProt)

Structure

• [PDB|3EUA]
• [AF|O32157]

Modification

• phosphorylated on Arg-48 [Pubmed|22517742]

[wiki|Localization]

• membrane associated [Pubmed|18763711]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• MGNA-A594 (yurP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/594 NBRP B. subtilis, Japan]
• BKE32610 ([gene|C87E74FB67B53DD1C68916AA121388C60CE66E92|frlB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATCCTTCACTCCTCGTT, downstream forward: _UP4_TGATCTGAAAATGAAGAACC
• BKK32610 ([gene|C87E74FB67B53DD1C68916AA121388C60CE66E92|frlB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATCCTTCACTCCTCGTT, downstream forward: _UP4_TGATCTGAAAATGAAGAACC

References

• 22517742,23175651,21815947,15556630,18083814,12618455,21347729,18763711,21398478,15378759,32983026