FolK

• Description: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
  
  

Gene name	folK
Synonyms
Essential	no
Product	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
Function	biosynthesis of folate
Gene expression levels in SubtiExpress: folK
Metabolic function and regulation of this protein in SubtiPathways: folK
MW, pI	18 kDa, 4.531
Gene length, protein length	501 bp, 167 aa
Immediate neighbours	folB, yazB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU00790

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate (according to Swiss-Prot)

• Protein family: HPPK family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1IM6 (from Escherichia coli, 47% identity, 61% similarity) PubMed

• UniProt: P29252

• KEGG entry: [3]

• E.C. number: 2.7.6.3

Expression and regulation

• Operon: pabB-pabA-pabC-sul-folB-folK-yazB-yacF-lysS PubMed

• Expression browser: folK PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Antoine de Saizieu, Pierre Vankan, Cassandra Vockler, Adolphus P G M van Loon
The trp RNA-binding attenuation protein (TRAP) regulates the steady-state levels of transcripts of the Bacillus subtilis folate operon.
Microbiology (Reading): 1997, 143 ( Pt 3);979-989
[PubMed:9084182] [WorldCat.org] [DOI] (P p)