Difference between revisions of "FolE"
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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of folate | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of folate | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22780 folE] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/phenyl_tyr_tryp.html Phe, Tyr, Trp], [http://subtiwiki.uni-goettingen.de/pathways/folate_biosynthesis.html Folate]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/phenyl_tyr_tryp.html Phe, Tyr, Trp], [http://subtiwiki.uni-goettingen.de/pathways/folate_biosynthesis.html Folate]''' | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mtrB]]'', ''[[hbs]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mtrB]]'', ''[[hbs]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22780 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22780 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22780 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:mtrA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:mtrA_context.gif]] | ||
Revision as of 13:09, 13 May 2013
- Description: GTP cyclohydrolase I
| Gene name | folE |
| Synonyms | mtrA |
| Essential | no |
| Product | GTP cyclohydrolase IA |
| Function | biosynthesis of folate |
| Gene expression levels in SubtiExpress: folE | |
| Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp, Folate | |
| MW, pI | 21 kDa, 6.335 |
| Gene length, protein length | 570 bp, 190 aa |
| Immediate neighbours | mtrB, hbs |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22780
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate (according to Swiss-Prot)
- Protein family: citrate synthase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): zinc-dependent enzyme PubMed
- Effectors of protein activity:
Database entries
- UniProt: P19465
- KEGG entry: [3]
- E.C. number: 3.5.4.16
Additional information
Expression and regulation
- Regulation: constitutive PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Banumathi Sankaran, Shilah A Bonnett, Kinjal Shah, Scott Gabriel, Robert Reddy, Paul Schimmel, Dmitry A Rodionov, Valérie de Crécy-Lagard, John D Helmann, Dirk Iwata-Reuyl, Manal A Swairjo
Zinc-independent folate biosynthesis: genetic, biochemical, and structural investigations reveal new metal dependence for GTP cyclohydrolase IB.
J Bacteriol: 2009, 191(22);6936-49
[PubMed:19767425]
[WorldCat.org]
[DOI]
(I p)
Basma El Yacoubi, Shilah Bonnett, Jessica N Anderson, Manal A Swairjo, Dirk Iwata-Reuyl, Valérie de Crécy-Lagard
Discovery of a new prokaryotic type I GTP cyclohydrolase family.
J Biol Chem: 2006, 281(49);37586-93
[PubMed:17032654]
[WorldCat.org]
[DOI]
(P p)
P Babitzke, P Gollnick, C Yanofsky
The mtrAB operon of Bacillus subtilis encodes GTP cyclohydrolase I (MtrA), an enzyme involved in folic acid biosynthesis, and MtrB, a regulator of tryptophan biosynthesis.
J Bacteriol: 1992, 174(7);2059-64
[PubMed:1551827]
[WorldCat.org]
[DOI]
(P p)
P Gollnick, S Ishino, M I Kuroda, D J Henner, C Yanofsky
The mtr locus is a two-gene operon required for transcription attenuation in the trp operon of Bacillus subtilis.
Proc Natl Acad Sci U S A: 1990, 87(22);8726-30
[PubMed:2123343]
[WorldCat.org]
[DOI]
(P p)
