Difference between revisions of "Fnr"

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(Extended information on the protein)
(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
 
 
* '''Operon:'''  
 
* '''Operon:'''  
 +
** ''[[narK]]-[[fnr]]'' {{PubMed|8846791}}
 +
** ''[[fnr]]'' {{PubMed|8846791}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
 +
** ''[[narK]]'': [[SigA]] {{PubMed|8846791}}
 +
** ''[[fnr]]'': [[SigA]] {{PubMed|8846791}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** expressed under anaerobic conditions ([[ResD]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10972836 PubMed]
+
** ''[[narK]]'': expressed under anaerobic conditions ([[Fnr]]) {{PubMed|8846791,16428414}}
 +
** ''[[fnr]]'': expressed under anaerobic conditions ([[ResD]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10972836 PubMed]
 
** repressed unless NO is present under anaerobic conditions ([[NsrR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16885456 PubMed]
 
** repressed unless NO is present under anaerobic conditions ([[NsrR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16885456 PubMed]
** expressed under anaerobic conditions ([[Fnr]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8846791,16428414 PubMed]
+
 
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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** [[Fnr]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8846791,16428414 PubMed]
 
** [[Fnr]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8846791,16428414 PubMed]
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 19:44, 3 February 2010

  • Description: transcriptional regulator of anaerobic genes

Gene name fnr
Synonyms
Essential no
Product transcription regulator (Crp family)
Function regulation of anaerobiosis, fermentation and overflow metabolism
MW, pI 27 kDa, 5.989
Gene length, protein length 714 bp, 238 aa
Immediate neighbours ywiC, narK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Fnr context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37310

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Genes/ operons controlled by Fnr

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: contains an iron-sulfur cluster
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • narK: expressed under anaerobic conditions (Fnr) PubMed
    • fnr: expressed under anaerobic conditions (ResD) PubMed
    • repressed unless NO is present under anaerobic conditions (NsrR) PubMed


  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456] [WorldCat.org] [DOI] (P p)

Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414] [WorldCat.org] [DOI] (P p)

M M Nakano, Y Zhu, M Lacelle, X Zhang, F M Hulett
Interaction of ResD with regulatory regions of anaerobically induced genes in Bacillus subtilis.
Mol Microbiol: 2000, 37(5);1198-207
[PubMed:10972836] [WorldCat.org] [DOI] (P p)

M M Nakano, P Zuber, P Glaser, A Danchin, F M Hulett
Two-component regulatory proteins ResD-ResE are required for transcriptional activation of fnr upon oxygen limitation in Bacillus subtilis.
J Bacteriol: 1996, 178(13);3796-802
[PubMed:8682783] [WorldCat.org] [DOI] (P p)

H Cruz Ramos, L Boursier, I Moszer, F Kunst, A Danchin, P Glaser
Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms.
EMBO J: 1995, 14(23);5984-94
[PubMed:8846791] [WorldCat.org] [DOI] (P p)