fmt

fmt
168

methionyl-tRNA formyltransferase

locus
BSU_15730
Molecular weight
34.48 kDa
pI
5.62
Protein length
Gene length
function
formylation of Met-tRNA(fMet)
product
methionyl-tRNA formyltransferase
essential
no
ec
2.1.2.9
synonyms
fmt, yloL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0223 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,646,999  1,647,952
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
inactivation of ''[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]'' suppresses the synthetic lethality of the ''[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA] [gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]'' double mutant [Pubmed|27983482]
essential according to Kobayashi et al. [Pubmed|12682299], but a deletion mutant was constructed by Cai et al. [Pubmed|27983482]
the mutant is defective for [wiki|biofilm formation], motility, and [wiki|sporulation]  [Pubmed|27983482]
the mutant exhibits increased sensitivity to metal ion excess and oxidative stress [Pubmed|27983482]
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The protein
Catalyzed reaction/ biological activity
(6S)-10-formyltetrahydrofolate + L-methionyl-tRNAfMet --> (6S)-5,6,7,8-tetrahydrofolate + H+ + N-formyl-L-methionyl-tRNAfMet (according to UniProt)
Protein family
fmt family (single member, according to UniProt)
Structure
[PDB|3RFO] (from ''Bacillus anthracis'', 69% identity, 89% similarity)
[AF|P94463]
Expression and Regulation
Operons
genes
[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[Pubmed|16964327]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16964327], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]→[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

2024-03-10 09:03:13

Jstuelk

54

d80326080a97255f868ab0fdec860faa54d5674c

244CB061EA23DF7106827650C1723DD251F3D15F

genes
[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|yloI]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[pubmed|22383849]
Open in new tab

[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]->[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

2024-03-28 14:50:08

Jstuelk

94

67080d1e476b4c1544f46abe5db9b45c3ccd93f3

2C39B75EDCD294B0CAC1DECB4007285A35FDAE1D

additional information
[protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
Biological materials
Mutant
BKE15730 ([gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAATACGATTCTCGTCA,  downstream forward: _UP4_GCCGGTGATGTGTTAGGAGT
BKK15730 ([gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAATACGATTCTCGTCA,  downstream forward: _UP4_GCCGGTGATGTGTTAGGAGT
References
8887566,19171795,27983482,12682299

06971E9AAB033E2878914A76645F99E97CE8A90A

Page visits: 3312

Time of last update: 2024-03-28 20:05:24

Author of last update: Christoph.elfmann