Difference between revisions of "FloT"

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** a ''[[floT]] [[floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency {{PubMed|22753055}}
 
** a ''[[floT]] [[floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency {{PubMed|22753055}}
 
** a ''[[floT]] [[floA]]'' double mutant has a [[sporulation]] defect, due to the lack of [[FtsH]] {{PubMed|22882210}}
 
** a ''[[floT]] [[floA]]'' double mutant has a [[sporulation]] defect, due to the lack of [[FtsH]] {{PubMed|22882210}}
 +
** a ''[[floT]]'' mutant displays a defective growth under oxygen-limiting conditions {{PubMed|25909364}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
 
** forms homo-oligomers {{PubMed|23651456}}
 
** forms homo-oligomers {{PubMed|23651456}}
 +
** oligomerization depends on the glutamate-alanine repeats {{PubMed|25909364}}
 
** [[YuaF]]-[[FloT]] {{PubMed|22753055}}
 
** [[YuaF]]-[[FloT]] {{PubMed|22753055}}
 
** [[FloT]]-[[FtsH]] {{PubMed|22882210}}
 
** [[FloT]]-[[FtsH]] {{PubMed|22882210}}
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** [[FloT]]-[[OppA]] {{PubMed|23651456}}
 
** [[FloT]]-[[OppA]] {{PubMed|23651456}}
 
** [[FloT]]-[[SdhA]] {{PubMed|23651456}}
 
** [[FloT]]-[[SdhA]] {{PubMed|23651456}}
 +
** [[FloT]]-[[ResE]] {{PubMed|25909364}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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** co-localizes with [[FloA]] and [[KinC]] in discrete foci membrane {{PubMed|20713508}}
 
** co-localizes with [[FloA]] and [[KinC]] in discrete foci membrane {{PubMed|20713508}}
 
** forms discrete focal structures in the cytoplasma membrane {{PubMed|22753055}}
 
** forms discrete focal structures in the cytoplasma membrane {{PubMed|22753055}}
 +
** 6 foci [[FloT]] of are detected in strain 3610 {{PubMed|25909364}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Mutant:'''
 
* '''Mutant:'''
 +
** JS152 (markerless), available in [[Daniel Lopez]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
 +
** GK38 (168 [[amyE]]::P[[floT-yfp]] (spc)), available in [[Daniel Lopez]]'s lab
 +
** JS280 (3610 [[amyE::floT-gfp]](spc)), available in [[Daniel Lopez]]'s lab
 +
** JS153 (3610 [[lacA::floT]]-mEos2 (mls)), available in [[Daniel Lopez]]'s lab
 +
** JS166 (3610 [[lacA::floT]]-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
  
−
* '''two-hybrid system:'''  
+
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 11:35, 30 April 2015

  • Description: similar to flotillin 1, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with YuaF) resistance to cefuroxime

Gene name floT
Synonyms yuaG, yuaH
Essential no
Product bacterial flotillin-like protein
Function involved in the control of membrane fluidity
Gene expression levels in SubtiExpress: floT
Interactions involving this protein in SubtInteract: FloT
MW, pI 55 kDa, 5.135
Gene length, protein length 1527 bp, 509 aa
Immediate neighbours yuaI, yuaF
Sequences Protein DNA DNA_with_flanks
Genetic context
YuaG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FloT expression.png















Categories containing this gene/protein

biofilm formation, membrane dynamics, membrane proteins sporulation/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

  • Locus tag: BSU31010

Phenotypes of a mutant

    • delayed onset of sporulation, reduced sporulation frequency
    • defect in motility PubMed
    • reduced protein secretion PubMed
    • a floT floA double mutant does not induce KinC-dependent biofilm formation upon addition of surfactin PubMed
    • a floT floA double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency PubMed
    • a floT floA double mutant has a sporulation defect, due to the lack of FtsH PubMed
    • a floT mutant displays a defective growth under oxygen-limiting conditions PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed upon cell wall stress (SigW) PubMed
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1215 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 236 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Daniel Lopez's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Marc Bramkamp, Daniel Lopez
Exploring the existence of lipid rafts in bacteria.
Microbiol Mol Biol Rev: 2015, 79(1);81-100
[PubMed:25652542] [WorldCat.org] [DOI] (I p)

Original publications

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