Difference between revisions of "FloT"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
** delayed onset of sporulation, reduced sporulation frequency
 
** delayed onset of sporulation, reduced sporulation frequency
** a ''[[floT]] [[yqfA]]'' double mutant does not induce [[KinC]]-dependent biofilm formation upon addition of surfactin {{PubMed|20713508}}
+
** defect in motility {{PubMed|22753055}}
 +
** a ''[[floT]] [[floA]]'' double mutant does not induce [[KinC]]-dependent biofilm formation upon addition of surfactin {{PubMed|20713508}}
 +
** a ''[[floT]] [[floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency {{PubMed|22753055}}
 
=== Database entries ===
 
=== Database entries ===
  
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
 
** [[SigW]]-dependent expression of ''[[fabF]]'' and the ''[[yuaF]]-[[floT]]-[[yuaI]]'' operon result in reduced membrane fluidity {{PubMed|21542858,22178969}}
 
** [[SigW]]-dependent expression of ''[[fabF]]'' and the ''[[yuaF]]-[[floT]]-[[yuaI]]'' operon result in reduced membrane fluidity {{PubMed|21542858,22178969}}
 +
** recruits [[YuaF]] to focal assemblies  {{PubMed|22753055}}
  
 
* '''Protein family:'''
 
* '''Protein family:'''
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* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
** [[YuaF]]-[[FloT]] (suspected based on conserved operon organisation and common function) {{PubMed|18696230,22178969}}
+
** [[YuaF]]-[[FloT]] {{PubMed|22753055}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
 
** membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
 
** membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
** co-localizes with [[YqfA]] and [[KinC]] in discrete foci membrane {{PubMed|20713508}}
+
** co-localizes with [[FloA]] and [[KinC]] in discrete foci membrane {{PubMed|20713508}}
 +
** forms focal structure in the cytoplasma membrane {{PubMed|22753055}}
  
 
=== Database entries ===
 
=== Database entries ===
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=References=
 
=References=
 
'''Additional publications:''' {{PubMed|22178969}}
 
'''Additional publications:''' {{PubMed|22178969}}
<pubmed>9987136,, 12107147, 18763711, 19383680  20713508 </pubmed>
+
<pubmed>9987136,22753055, 12107147, 18763711, 19383680  20713508 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:25, 6 July 2012

  • Description: similar to flotillin 1, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with YuaF) resistance to cefuroxime

Gene name floT
Synonyms yuaG, yuaH
Essential no
Product bacterial flotillin-like protein
Function involved in the control of membrane fluidity
Interactions involving this protein in SubtInteract: FloT
MW, pI 55 kDa, 5.135
Gene length, protein length 1527 bp, 509 aa
Immediate neighbours yuaI, yuaF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YuaG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FloT expression.png
















Categories containing this gene/protein

biofilm formation, membrane dynamics, membrane proteins sporulation/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

  • Locus tag: BSU31010

Phenotypes of a mutant

    • delayed onset of sporulation, reduced sporulation frequency
    • defect in motility PubMed
    • a floT floA double mutant does not induce KinC-dependent biofilm formation upon addition of surfactin PubMed
    • a floT floA double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed upon cell wall stress (SigW) PubMed
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Felix Dempwolff, Heiko M Möller, Peter L Graumann
Synthetic motility and cell shape defects associated with deletions of flotillin/reggie paralogs in Bacillus subtilis and interplay of these proteins with NfeD proteins.
J Bacteriol: 2012, 194(17);4652-61
[PubMed:22753055] [WorldCat.org] [DOI] (I p)

Daniel López, Roberto Kolter
Functional microdomains in bacterial membranes.
Genes Dev: 2010, 24(17);1893-902
[PubMed:20713508] [WorldCat.org] [DOI] (I p)

Catriona Donovan, Marc Bramkamp
Characterization and subcellular localization of a bacterial flotillin homologue.
Microbiology (Reading): 2009, 155(Pt 6);1786-1799
[PubMed:19383680] [WorldCat.org] [DOI] (P p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

X Huang, A Gaballa, M Cao, J D Helmann
Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W.
Mol Microbiol: 1999, 31(1);361-71
[PubMed:9987136] [WorldCat.org] [DOI] (P p)