Difference between revisions of "FloT"

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(Extended information on the protein)
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
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* '''[[SubtInteract|Interactions]]:'''  
  
* '''Localization:''' membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed], co-localizes with [[YqfA]] and [[KinC]] in discrete foci membrane {{PubMed|20713508}}
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* '''[[Localization]]:'''
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** membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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** co-localizes with [[YqfA]] and [[KinC]] in discrete foci membrane {{PubMed|20713508}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 20:03, 11 August 2011

  • Description: similar to flottilin 1, orchestration of physiological processes in lipid microdomains

Gene name floT
Synonyms yuaG, yuaH
Essential no
Product unknown
Function early stages of sporulation
MW, pI 55 kDa, 5.135
Gene length, protein length 1527 bp, 509 aa
Immediate neighbours yuaI, yuaF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YuaG context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

biofilm formation, membrane dynamics, membrane proteins sporulation/ other, cell envelope stress proteins (controlled by SigM, W, X, Y)

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

  • Locus tag: BSU31010

Phenotypes of a mutant

    • delayed onset of sporulation, reduced sporulation frequency
    • a floT yqfA double mutant does not induce KinC-dependent biofilm formation upon addition of surfactin PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed upon cell wall stress (SigW) PubMed
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Daniel López, Roberto Kolter
Functional microdomains in bacterial membranes.
Genes Dev: 2010, 24(17);1893-902
[PubMed:20713508] [WorldCat.org] [DOI] (I p)

Catriona Donovan, Marc Bramkamp
Characterization and subcellular localization of a bacterial flotillin homologue.
Microbiology (Reading): 2009, 155(Pt 6);1786-1799
[PubMed:19383680] [WorldCat.org] [DOI] (P p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

X Huang, A Gaballa, M Cao, J D Helmann
Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W.
Mol Microbiol: 1999, 31(1);361-71
[PubMed:9987136] [WorldCat.org] [DOI] (P p)