Difference between revisions of "FloA"

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(Biological materials)
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** JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab  
 
** JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab  
  
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Daniel Lopez]]'s lab
+
* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) for FloA {{PubMed|25909364}}, available in [[Daniel Lopez]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 11:41, 30 April 2015

  • Description: flottilin-like protein (in addition to FloT), resistence protein (against sublancin), accessory role in resistance to cefuroxime

Gene name floA
Synonyms yqfA
Essential no
Product flottilin-like protein
Function resistance against sublancin
Gene expression levels in SubtiExpress: floA
Interactions involving this protein in SubtInteract: FloA
MW, pI 35 kDa, 4.917
Gene length, protein length 993 bp, 331 aa
Immediate neighbours yqfB, yqeZ
Sequences Protein DNA DNA_with_flanks
Genetic context
YqfA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YqfA expression.png















Categories containing this gene/protein

membrane dynamics, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

  • Locus tag: BSU25380

Phenotypes of a mutant


Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0365 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:


Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 3975 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3635 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
    • GK192 (3610 lacA::PfloT-yfp(mls)), available in Daniel Lopez's lab
    • JS136 (3610 amyE::floT-gfp(spc)), available in Daniel Lopez's lab
    • JS134 (3610 lacA::floA-mEos2 (mls)), available in Daniel Lopez's lab
    • JS167 (3610 lacA::floA-PAmCherry (mls)), available in Daniel Lopez's lab
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) for FloA PubMed, available in Daniel Lopez's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Marc Bramkamp, Daniel Lopez
Exploring the existence of lipid rafts in bacteria.
Microbiol Mol Biol Rev: 2015, 79(1);81-100
[PubMed:25652542] [WorldCat.org] [DOI] (I p)

Original publications