fliG

168

flagellar basal-body protein, membrane anchor of the basal body, flagellar motor switch protein, physically transduces force from [protein|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA] to the rotation of [protein|EB815705133E3318F655F6024B7D9BA587FD1DDA|fliF]

locus: BSU_16220

Molecular weight: 38.04 kDa

pI: 4.46

Protein length: 338 aa

Gene length: 1017 bp

function: movement and chemotaxis

product: flagellar C ring protein

essential: no

synonyms: fliG, flaA15

List of homologs in different organisms, belongs to COG1536 (Galperin et al., 2021)

Gene

Coordinates: 1,694,119  1,695,135

Phenotypes of a mutant

• mucoid phenotype due to the overproduction of poly-gamma-glutamate [Pubmed|24296669]
• no secretion of [protein|F03144BF8A187C8931938A21433431B8961E8EE7|flgM], permanent inhibition of [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD] [Pubmed|25313396]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

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The protein

Catalyzed reaction/ biological activity

• physically transduces force from [protein|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA] to the rotation of [protein|EB815705133E3318F655F6024B7D9BA587FD1DDA|fliF]

Protein family

• FliG family (single member, according to UniProt)

Structure

• [PDB|3HJL] (from ''Aquifex aeolicus'', 31% identity) [Pubmed|20676082]
• [AF|P23448]

Effectors of protein activity

• interaction with [protein|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE] prevents energetization of the flagellum by [protein|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]-[protein|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB] [Pubmed|18566286]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• BKE16220 ([gene|8DDCC3D139ACCB635BF014946E1282A72D535390|fliG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACGTCTCGCCATTTCTAATT,  downstream forward: _UP4_TAATATCATTAAACAAGAAT
• BKK16220 ([gene|8DDCC3D139ACCB635BF014946E1282A72D535390|fliG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ACGTCTCGCCATTTCTAATT,  downstream forward: _UP4_TAATATCATTAAACAAGAAT

References

Reviews

• 30718302

Original Publications

• 1905667,9657996,8157612,15175317,14651647,18566286,17850253,24296669,25313396,24386445,25733861,26244495,20676082,29196522,30455280,35638827