Difference between revisions of "FliF"

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|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16210 fliF]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16210 fliF]
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FliF FliF]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 59 kDa, 4.37   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 59 kDa, 4.37   
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
* mucoid phenotype due to the overproduction of poly-gamma-glutamate {{PubMed|24296669}}
 
* mucoid phenotype due to the overproduction of poly-gamma-glutamate {{PubMed|24296669}}
 +
* no secretion of [[FlgM]], permanent inhibition of [[SigD]] {{PubMed|25313396}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
 +
** [[FliF]]-[[FliG]] {{PubMed|25313396}}
  
* '''[[Localization]]:''' cell membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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* '''[[Localization]]:'''  
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** cell membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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=References=
 
=References=
  
<pubmed>1905667,17850253,14651647,18763711, 9657996,8157612,15175317 23190039 24296669</pubmed>
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<pubmed>1905667,17850253,14651647,18763711, 9657996,8157612,15175317 23190039 24296669 25313396</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:01, 27 October 2014

  • Description: flagellar basal-body M-ring protein, membrane anchor of the basal body

Gene name fliF
Synonyms
Essential no
Product flagellar basal-body M-ring protein
Function movement and chemotaxis
Gene expression levels in SubtiExpress: fliF
Interactions involving this protein in SubtInteract: FliF
MW, pI 59 kDa, 4.37
Gene length, protein length 1608 bp, 536 aa
Immediate neighbours fliE, fliG
Sequences Protein DNA DNA_with_flanks
Genetic context
FliF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FliF expression.png















Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16210

Phenotypes of a mutant

  • mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed
  • no secretion of FlgM, permanent inhibition of SigD PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: fliF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 68 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 595 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References