FlhO

• Description: flagellar basal-body rod protein, required for hook assembly
  
  

Gene name	flhO
Synonyms	flgG, yvyA
Essential	no
Product	flagellar basal-body rod protein
Function	motility and chemotaxis
MW, pI	29 kDa, 4.733
Gene length, protein length	810 bp, 270 aa
Immediate neighbours	flhP, mbl
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU36400

Phenotypes of a mutant

• lack of SigD-dependent gene expression (no hag expression), no motility PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: assembly of the flagellar hook PubMed

• Protein family: flagella basal body rod proteins family (according to Swiss-Prot)

• Paralogous protein(s): FlgE, FlhP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (no signal peptide) PubMed

Database entries

• Structure:

• UniProt: P39752

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • usd-spoIIID-mbl-flhO-flhP PubMed
  • flhO-flhP PubMed

• Expression browser: flhO PubMed

• Sigma factor:
  • usd: SigE PubMed
  • flhO: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Masakuni Serizawa, Hiroki Yamamoto, Hirotake Yamaguchi, Yasutaro Fujita, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.
Gene: 2004, 329;125-36
[PubMed:15033535] [WorldCat.org] [DOI] (P p)

A Decatur, M T McMurry, B N Kunkel, R Losick
Translation of the mRNA for the sporulation gene spoIIID of Bacillus subtilis is dependent upon translation of a small upstream open reading frame.
J Bacteriol: 1997, 179(4);1324-8
[PubMed:9023218] [WorldCat.org] [DOI] (P p)

Y Abhayawardhane, G C Stewart
Bacillus subtilis possesses a second determinant with extensive sequence similarity to the Escherichia coli mreB morphogene.
J Bacteriol: 1995, 177(3);765-73
[PubMed:7836311] [WorldCat.org] [DOI] (P p)