Difference between revisions of "FlhG"

From SubtiWiki
Jump to: navigation, search
(Extended information on the protein)
Line 85: Line 85:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
Line 94: Line 94:
  
 
* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
 +
** forms homodimers {{PubMed|25733861}}
 
** [[FlhG]]-[[FlhF]] ([[FlhG]] binds the GTP-bound homodimer of [[FlhF]]) {{PubMed|22056770}}
 
** [[FlhG]]-[[FlhF]] ([[FlhG]] binds the GTP-bound homodimer of [[FlhF]]) {{PubMed|22056770}}
 
** [[FlhG]]-([[FliY]]-[[FliM]])-[[FliG]] {{PubMed|25733861}}
 
** [[FlhG]]-([[FliY]]-[[FliM]])-[[FliG]] {{PubMed|25733861}}
  
* '''[[Localization]]:''' cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
+
* '''[[Localization]]:''' cytoplasm (homogeneous) and flagellar basal body {{PubMed|25733861,16479537}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 12:16, 4 March 2015

  • Description: GTPase activating protein, activates FlhF, control of flagellar basal body position

Gene name flhG
Synonyms ylxH
Essential no
Product GTPase activating protein
Function activation of FlhF
Gene expression levels in SubtiExpress: flhG
Interactions involving this protein in SubtInteract: FlhG
MW, pI 33 kDa, 9.648
Gene length, protein length 894 bp, 298 aa
Immediate neighbours flhF, cheB
Sequences Protein DNA DNA_with_flanks
Genetic context
YlxH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YlxH expression.png















Categories containing this gene/protein

motility and chemotaxis, resistance against toxins/ antibiotics

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16410

Phenotypes of a mutant

  • susceptible to acriflavine and ethidium bromide, and severe growth inhibition as surfactin concentration increased up to 100ug/ml.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binds FlhF and stimulates its GTPase activity PubMed
    • controls the distance between the flagellar bodies PubMed
  • Protein family: View classification (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jan S Schuhmacher, Florian Rossmann, Felix Dempwolff, Carina Knauer, Florian Altegoer, Wieland Steinchen, Anja K Dörrich, Andreas Klingl, Milena Stephan, Uwe Linne, Kai M Thormann, Gert Bange
MinD-like ATPase FlhG effects location and number of bacterial flagella during C-ring assembly.
Proc Natl Acad Sci U S A: 2015, 112(10);3092-7
[PubMed:25733861] [WorldCat.org] [DOI] (I p)

Serena Mordini, Cecilia Osera, Simone Marini, Francesco Scavone, Riccardo Bellazzi, Alessandro Galizzi, Cinzia Calvio
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis.
PLoS One: 2013, 8(12);e85065
[PubMed:24386445] [WorldCat.org] [DOI] (I e)

Sarah B Guttenplan, Sidney Shaw, Daniel B Kearns
The cell biology of peritrichous flagella in Bacillus subtilis.
Mol Microbiol: 2013, 87(1);211-29
[PubMed:23190039] [WorldCat.org] [DOI] (I p)

Gert Bange, Nico Kümmerer, Przemyslaw Grudnik, Robert Lindner, Georg Petzold, Dieter Kressler, Ed Hurt, Klemens Wild, Irmgard Sinning
Structural basis for the molecular evolution of SRP-GTPase activation by protein.
Nat Struct Mol Biol: 2011, 18(12);1376-80
[PubMed:22056770] [WorldCat.org] [DOI] (I e)

Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317] [WorldCat.org] [DOI] (P p)

Daniel B Kearns, Frances Chu, Rivka Rudner, Richard Losick
Genes governing swarming in Bacillus subtilis and evidence for a phase variation mechanism controlling surface motility.
Mol Microbiol: 2004, 52(2);357-69
[PubMed:15066026] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996] [WorldCat.org] [DOI] (P p)

L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612] [WorldCat.org] [DOI] (P p)