flhF

168

signal recognition particle-like GTPase, placement and assembly of flagella, control of basal body position

locus: BSU_16400

Molecular weight: 40.99 kDa

pI: 8.72

Protein length: 366 aa

Gene length: 1101 bp

function: placement and assembly of polar flagella

product: signal recognition particle-like GTPase

essential: no

synonyms: flhF

List of homologs in different organisms, belongs to COG1419 (Galperin et al., 2021)

Gene

Coordinates: 1,709,747 → 1,710,847

Phenotypes of a mutant

• inactivation of ''[gene|BB6F5D7463EF96E2C6344D0BC30A227C5E3B5817|flhF]'' confers resistance to high concentrations of Zn(II) [Pubmed|27935957]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

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The protein

Catalyzed reaction/ biological activity

• GTPase activity  [Pubmed|22056770]
• placement of the first flagellar protein, [protein|EB815705133E3318F655F6024B7D9BA587FD1DDA|fliF] [Pubmed|22056770]

Protein family

• GTP-binding SRP family (with [protein|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh] and [protein|D519765BF5261E48512CE86245BD7B733F188D4A|ftsY], according to UniProt)

Structure

• [PDB|2PX3] (complexed with GTP-Mg2+) [Pubmed|17699634]
• [PDB|3SYN] (complex with its activator [protein|D6D34E68FB3BAAACB533F98290FD363A5B00B2C6|flhG]) [Pubmed|22056770]
• [AF|Q01960]

Effectors of protein activity

• interaction with [protein|D6D34E68FB3BAAACB533F98290FD363A5B00B2C6|flhG] activates the GTPase activity of [protein|BB6F5D7463EF96E2C6344D0BC30A227C5E3B5817|flhF] [Pubmed|22056770]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• 1A925 ( ''flhF''::''cat''), [Pubmed|15317803], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A925&Search=1A925 BGSC]
• BKE16400 (Δ[gene|BB6F5D7463EF96E2C6344D0BC30A227C5E3B5817|flhF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAAATATCCACCACTCC,  downstream forward: _UP4_CTGTGCAGATGAACAGATAT
• BKK16400 (Δ[gene|BB6F5D7463EF96E2C6344D0BC30A227C5E3B5817|flhF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAAATATCCACCACTCC,  downstream forward: _UP4_CTGTGCAGATGAACAGATAT

References

Reviews

• 26195616

Original Publications

• 1447978,17699634,17850253,15317803,14651647,9657996,8157612,15175317,22056770,23190039,24386445,27935957,35638827